[HTML] from nih.govP Laksanalamai, DL Maeder… - Journal of bacteriology, 2001 - Am Soc Microbiol
... In order to determine whether Pfu-sHSP is regulated in response to heat shock, we obtained polyclonal antiserum against Pfu-sHSP by immunization of a rabbit with purified, recombinant Pfu-sHSP (
BioWorld,
Dublin,
Ohio).
... Cited by 37 -
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[HTML] from jbc.orgX Li, B Alvarez, JR Casey, RAF Reithmeier… - Journal of Biological …, 2002 - ASBMB
... Cruz, CA). Rabbit anti-His tag antibody was purchased from
BioWorld,
Dublin,
OH. Conjugated antibodies were from Jackson ImmunoResearch (Mississauga, Ont.). Ni-NTA-agarose resin was from Qiagen (Valencia, CA). α-Casein
... Cited by 84 -
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[HTML] from pnas.orgD Chereau, F Kerff, P Graceffa… - Proceedings of the …, 2005 - National Acad Sciences
... Materials and Methods. Proteins and Peptides. Actin was prepared and labeled with acrylodan as described in ref. 11. Ultrapure-grade bovine pancreatic DNase I was purchased from
BioWorld (
Dublin,
OH). Peptides corresponding
... Cited by 81 -
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JF Harms, DR Welch, RS Samant… - Clinical and …, 2004 - Springer
... Osteoclast-like cells were detached from culture dishes using a non-enzymatic cell dissociation buffer (Gibco BRL, Rockville, Maryland), then transferred into 24-well cell culture plates containing 15-mm diameter borosilicate glass coverslips (
Bioworld,
Dublin,
Ohio) that were
... Cited by 76 -
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C Noriega, DJ Hassett… - Current microbiology, 2005 - Springer
... In addition, complete anaerobiosis was ensured by the addition of 2% (w/v) Oxyrase enzyme (Oxyrase, Mansfield,
OH). Antibiotics from
Bioworld (
Dublin,
OH) were used in E. coli cultures at the following Table 1. Strains and plasmids Strains
... Cited by 7 -
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S Tang, NS Hettiarachchy… - Journal of Food …, 2003 - interscience.wiley.com
... The slurries containing en- zymes were mixed, and those treated with amylase and viscozyme were incubated at 45 °C and shaken at 200 rpm in the Micro-Envi- ron shaker (
Bio-
WORLD,
Dublin,
Ohio, USA) for 3.5 h, while the ones with celluclast were incubated at 55 °C and
... Cited by 9 -
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B Chen, MU Mayer… - Biochemistry, 2005 - ACS Publications
... described (27). The peptide C28W (LRRGQILWFRGLNRIQTQIRVVNAFRSS) was synthesized, purified, and confirmed by mass spectrometry to be greater than 95% pure by
BioWORLD (
Dublin,
OH). Hydrogen peroxide (H
... Cited by 24 -
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[HTML] from nih.govSR Heimer… - Journal of Bacteriology, 2001 - Am Soc Microbiol
... Preparation of monoclonal antibodies to UreC and UreD. Purified UreC and UreD proteins embedded in SDS-polyacrylamide were supplied to
BioWorld Laboratories (
Dublin,
Ohio) for the production of monoclonal antibodies.
... Cited by 20 -
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[HTML] from jbc.orgDO Lambeth, KN Tews, S Adkins, D Frohlich… - Journal of Biological …, 2004 - ASBMB
... Western Blots—Antipeptide antibodies to SCS subunits were prepared by immunizing chicken (α subunit, Gallus Immunotech, Ontario, Canada) or rabbit (both β subunits,
Bioworld,
Dublin,
OH) using the following sequences: KEAHVDVKFQLPI, A-β C-terminal
... Cited by 26 -
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All 4 versions
X Li, Y Liu, CM Kay, W Müller-Esterl… - Biochemistry, 2003 - ACS Publications
... Louis, MO). Rabbit anti-His-tag antibody was purchased from
BioWorld,
Dublin,
OH. Conjugated antibodies were from Jackson ImmunoResearch (Mississauga, Ontario, Canada). Ni-NTA−agarose resin was from Qiagen (Valencia, CA).
... Cited by 28 -
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[HTML] from aspetjournals.orgDD Vakharia, N Liu, R Pause, M Fasco… - Drug Metabolism and …, 2001 - ASPET
... Sigma. After a 45-min incubation at 37°C in a CO 2 incubator, 75 µl of culture media from each well was transferred to white, opaque 96-well plates supplied by
Bioworld Lab Essentials (
Dublin,
OH). -Glucuronidase/sulfatase
... Cited by 54 -
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[HTML] from diabetesjournals.orgK Kelemen, DR Wegmann… - Diabetes, 2001 - Am Diabetes Assoc
... A series of synthetic 20mer peptides, each overlapping by 10 amino acids and spanning two distinct epitope regions on the phogrin C terminus, were obtained from
BioWorld (
Dublin,
OH) (Table 1). They were introduced into T-cell proliferation assays as aqueous solutions at 30
... Cited by 16 -
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[PDF] from biologists.orgL Williams, SP Grigg, M Xie, S Christensen… - …, 2005 - dev.biologists.org
... GUS staining The GUS staining reaction and subsequent tissue embedding and sectioning were performed as described previously (Sieburth and Meyerowitz, 1997) using 2 mM 5-bromo-4-chloro-3-indolyl-β-D glucoronide (X-GLUC;
Bioworld,
Dublin,
OH, USA).
... Cited by 117 -
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A Dibas, G Prasanna… - Journal of Ocular Pharmacology …, 2005 - liebertonline.com
... METHODS Materials E64, pepstatin, phosphoramdion, phenanthro- line, and thiorphan were purchased from Sigma- Aldrich Co. (St. Louis, MO). Big ET-116–37 was synthesized by
BioWorld Labs (
Dublin,
Ohio). Polyvinylidene difluoride (PVDF) transfer mem-
... Cited by 3 -
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All 3 versions
S Katengam, JM Crane… - TAG Theoretical and Applied …, 2002 - Springer
... running buffer (0.045 M Tris borate and 0.001 M EDTA, pH 8.0) at 60 W for 2 h, transferred to chromatographic paper (3 MM) (Fisher Scientific, Pittsburgh, Pa., USA), dried on a gel-dryer under vacuum at 80°C for 2 h, and exposed to X-ray film (
Bioworld,
Dublin,
Ohio, USA) at
... Cited by 6 -
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[HTML] from jbc.orgD Locke, C Liu, X Peng, H Chen… - Journal of Biological …, 2003 - ASBMB
... nonanoylN-methylglucamide. For gold staining membranes were washed 3× for 10 min in phosphate-buffered saline, 0.5% Tween and then developed using membragold (
Bioworld,
Dublin,
OH). Fluorescence Spectroscopy.
... Cited by 29 -
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[HTML] from nih.govCE Cameron, NL Brouwer, LM Tisch… - Infection and …, 2005 - Am Soc Microbiol
... Synthetic peptides. Peptides were synthesized by
Bio-
WORLD (
Dublin,
OH) using Fmoc (9-fluorenylmethoxycarbonyl)-based solid phase chemistry. Peptides were purified using high-performance liquid chromatography, and
... Cited by 20 -
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J Dietrich, M Noble… - Glia, 2002 - interscience.wiley.com
... To study the differentiation potential of human NEP and A2B5 cells, the different populations were plated on fibronectin/laminin-coated coverslips (
Bio-
World,
Dublin,
OH) in 24-well plates (Nunc) at a density of 103 cells/well and were exposed to differentiation inducing
... Cited by 53 -
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AB Lopez, K Sener, EL Jarroll… - Molecular and biochemical …, 2003 - Elsevier
... proteins by standard procedures. The polyclonal antibodies directed against GNA, AGM, and UAP were generated by Rockland (Gilbertsville, PA); the UAE antiserum was prepared by
Bio-
World (
Dublin,
OH). The IgG fraction of
... Cited by 18 -
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All 4 versions
[HTML] from jbc.orgJD Weimar, CC DiRusso, R Delio… - Journal of Biological …, 2002 - ASBMB
... Antisera were prepared against purified FadD using a commercial vendor (
BioWorld,
Dublin,
OH). SelectedfadD alleles were subcloned as cassettes into pN3576 for expression and purification of mutant forms of the enzyme as detailed under “Results.”. Western Blotting.
... Cited by 37 -
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[HTML] from molbiolcell.orgYS Bahn, K Kojima, GM Cox… - Molecular biology of the …, 2005 - Am Soc Cell Biol
... D. The gel-extracted HOG1 disruption cassette was precipitated onto 600 µg of gold microcarrier beads (0.8-µm,
bioWORLD,
Dublin,
OH) and biolistically transformed into the prototrophic serotype A and D wild-type strains as described previously (Davidson et al., 2000 Go ).
... Cited by 64 -
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All 7 versions
[PDF] from usda.govAL Dhanaraj, JP Slovin… - Plant Science, 2005 - Elsevier
... Immunoreactive bands were detected on X-ray film (Sterling
Bioworld,
Dublin,
OH, USA) using a chemiluminescent immunoblot detection system with CDP-Star substrate (Western Star System, Tropix, Applied Biosystems, Bedford, MA, USA).
... Cited by 24 -
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All 5 versions
SE Alway, H Degens… - The Journals …, 2003 - biomedgerontology.oxfordjournals. …
... Caspase 3,7,10 and caspase 8 were measured using commercial colorimetric apoptosis assay kits (
bioWorld,
Dublin,
OH), using the free dye of 7-amino-4-trifluromethyl coumarin (AFC) as a standard, according to the procedures outlined by the manufacturer.
... Cited by 53 -
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[HTML] from jbc.orgL He, MJJ Ronis… - Journal of Biological Chemistry, 2002 - ASBMB
... This peptide was checked for matching sequences in the Entrez protein sequence data library (NCBI) and found to be unique. An antisera against this sequence was produced in rabbits by
Bioworld (
Dublin,
OH). Western Immunoblot Analysis.
... Cited by 14 -
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[HTML] from molbiolcell.orgR Pai, AS Tarnawski… - Molecular biology of the cell, 2004 - Am Soc Cell Biol
... triplicate. Potential cytotoxic effect of various transfections was determined by assessing lactose dehydrogenase (LDH) release into the cell culture medium using a Cytoscan LDH-cytotoxicity assay kit (
Bioworld,
Dublin,
OH).
... Cited by 44 -
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SD Webb, JL Cleland… - Journal of …, 2002 - interscience.wiley.com
... Louis, MO. The Non- Interfering Protein Assay© was purchased from
Bioworld Laboratory Essentials,
Dublin,
OH, and used according to the manufacturer's Protocol I instructions. All reagents were ACS reagent grade or higher quality.
... Cited by 29 -
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[PDF] from ucla.eduSF Cummins, AE Nichols, K Rajarathnam… - Peptides, 2004 - Elsevier
... Statistical significance was assessed by chi-square analysis. In each case, test animals were choosing between a stimulus in one arm and no stimulus in another. 2.2. Peptide synthesis. Two peptides were synthesized by
bioWORLD (
Dublin,
OH).
... Cited by 16 -
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[HTML] from ukpmc.ac.ukD Chereau, F Kerff, P Graceffa… - Proceedings of the …, 2005 - ukpmc.ac.uk
... Proteins and Peptides. Actin was prepared and labeled with acrylodan as described in ref. 11. Ultrapure-grade bovine pancreatic DNase I was purchased from
BioWorld (
Dublin,
OH). Peptides corresponding to WASP 430–458
... Related articles
[HTML] from oxfordjournals.orgDC Spink, BH Katz, MM Hussain… - …, 2003 - Oxford Univ Press
... well). After 30 min at 37°C, 75 µl of the medium was transferred to a white, opaque 96-well plate (
Bioworld Lab Essentials,
Dublin,
OH). Ethanol (200 µl/well) was added, and the plate was centrifuged at 1500 g for 10 min. Fluorescence
... Cited by 38 -
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[HTML] from jbc.orgZ Zou, CC DiRusso, V Ctrnacta… - Journal of Biological Chemistry, 2002 - ASBMB
... The purified peptide was used as the antigen for the production of polyclonal anti-Fat1p serum using a commercial vendor (
BioWorld,
Dublin,
OH). Methods for SDS-polyacrylamide gel electrophoresis and Western blotting are standard and have been previously described (45).
... Cited by 58 -
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[HTML] from jimmunol.orgK Kelemen, PA Gottlieb, AL Putnam… - The Journal of …, 2004 - Am Assoc Immnol
... Synthetic peptides. Peptides were generated by custom synthesis (
BioWorld,
Dublin,
OH), purified by HPLC (>95%), and verified by mass spectrometry. The 15-mer phogrin peptides 2 and 7 are targeted by previously generated
... Cited by 12 -
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[CITATION] Id2 expression during apoptosis and satellite cell activation in unloaded and loaded quail skeletal muscles
Free from PublisherSE Alway, JK Martyn, J Ouyang… - American Journal of …, 2003 - Am Physiological Soc
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[PDF] from usda.govAL Dhanaraj, JP Slovin… - Plant Science, 2004 - Elsevier
... kit for 30 min and hybridized overnight at 42 °C. The following day, blots were washed twice (15 min, 42 °C) with Low Stringency wash solution from the NorthernMax™ kit and then exposed to X-ray film (Sterling
Bioworld,
Dublin,
OH, USA) with intensifying screens at −80 °C.
... Cited by 34 -
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[PDF] from ccf.orgA Agarwal, SSR Allamaneni… - Reproductive BioMedicine Online, 2004 - Elsevier
... 12 a Single tube 1000 molecules of luciferase, 5350 Zylux Corporation, Oak Ridge, 6 orders TN, USA Traithler Single tube 1 10 pg ATP, 7 orders 6000 Bioscan, Washington, DC, USA Zylux FB15 a Single tube 1000 molecules of luciferase, 7450
Bio-
World,
Dublin,
OH, USA >6
... Cited by 16 -
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All 7 versions
RP McCord, JN Yukich… - Cell motility and the …, 2005 - Wiley Online Library
... illumination. Measurements were made using 5–6 μL aliquots of appropriately dilute cells placed in 1-mm slide wells (
BioWorld,
Dublin,
OH) with affixed cover- slips and were taken within 10 min of slide preparation. Swimming
... Cited by 6 -
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[PDF] from tntech.eduGO Osuji, AS Mangaroo… - SAAS Bull. Biochem. …, 2001 - orgs.tntech.edu
... Primary antibody against the maize GDH was prepared in rabbits (
BioWorld Lab. Services,
Dublin,
Ohio, USA). Pre-immune serum was taken before the rabbits were immunized. Bleeds were collected on the 3rd, 6*, 9"1 and 14* weeks. Antisera titre was monitored by ELISA.
... Cited by 5 -
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V Orbović, G Pasquali… - … of Temperate Fruit Crops and Tropical …, 2005 - ISHS
... Linearized pPZP212 vector and 2143 bp fragment obtained in the digestion of pLMNC95 were gel purified using GeneEXITTM DNA Isolation Kit (
Bio-
World,
Dublin,
OH). pT-LAB21 (Fig. 1) was obtained by ligating purified fragment from pLMNC95 into the SmaI site of pPZP212.
... Related articles
[HTML] from sgmjournals.orgW Schreiber, KD Stone, MA Strong… - …, 2002 - Soc General Microbiol
... using standard procedures. Ascites was prepared commercially (
BioWorld,
Dublin,
OH) and purified using an EZ Sep kit (Amersham Pharmacia Biotech), according to the manufacturer's instructions. Mouse polyclonal anti-BfpU
... Cited by 12 -
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HAFP Monroy - Neuroimmunomodulation, 2001 - content.karger.com
... Briefly, 96-well Nunc-Immuno Maxi- Sorp Surface microtiter plates (
BioWorld Laboratory Essentials,
Dublin,
Ohio, USA) were coated with 1.5–5.0 Ìg/ml (depending on the cytokine) of capture monoclonal-specific antibody in coating buffer (0.1 M carbonate, pH 9.5).
... Cited by 11 -
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LD Joh, T Wroblewski, NN Ewing… - Biotechnology and …, 2005 - Wiley Online Library
... The disks were covered with 10 mL of an aqueous solution containing 5% of 10 mg/mL X-Gluc (
Bio-
World,
Dublin,
OH) in dimethyl sulfoxide (Sigma); 100 mM sodium phosphate at pH 7.0; 10 mM EDTA (Bio-Rad, Hercules, CA); 0.5 mM potassium ferricyanide; 0.5 mM potassium
... Cited by 20 -
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[PDF] from asas.orgCM Murrieta, EJ Scholljegerdes, BW Hess, DC Rule… - EVALUATION, 2005 - asas.org
... using 18s mRNA. Membrane transfer and autoradiography were conducted according to Lee et al., (2002) except that a 45 min exposure to X-ray film was used (Sterling RX-B®,
bio-
World,
Dublin,
OH). Films were digitized and
... Cited by 2 -
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[CITATION] Increased susceptibility to Pseudomonas aeruginosa infection under hindlimb-unloading conditions
[HTML] from shouxi.net Free from PublisherH Aviles, T Belay, K Fountain… - Journal of Applied …, 2003 - Am Physiological Soc
Cited by 19 -
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R Samarakoon… - Journal of cellular physiology, 2003 - Wiley Online Library
... antibodies. Membranes were stripped with Western Stripper kit (
Bioworld,
Dublin,
OH) according to the manufacturer's instructions and reprobed with Myc antibodies (1:1,000) to quantify exogenous ERK2 levels. To evaluate
... Cited by 7 -
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A Molina, J Veramendi… - Virology, 2005 - Elsevier
... The 2L21 peptide (MSDGAVQPDGGQPAVRNERATG) and the control peptide WNSTAFHQTLQDPR (amino acids 122–135 of the pre-S2 region of the hepatitis B virus surface antigen) were synthesized by
Bioworld (
Dublin,
Ohio, USA).
... Cited by 36 -
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All 5 versions
[CITATION] Active hexose correlated compound enhances resistance to Klebsiella pneumoniae infection in mice in the hindlimb-unloading model of spaceflight …
[HTML] from shouxi.net Free from PublisherH Aviles, T Belay, K Fountain… - Journal of Applied …, 2003 - Am Physiological Soc
Cited by 25 -
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[HTML] from ukpmc.ac.ukR Pai, AS Tarnawski… - Molecular Biology of the Cell, 2004 - ukpmc.ac.uk
... triplicate. Potential cytotoxic effect of various transfections was determined by assessing lactose dehydrogenase (LDH) release into the cell culture medium using a Cytoscan LDH-cytotoxicity assay kit (
Bioworld,
Dublin,
OH).
... Related articles
[HTML] from pnas.orgC Tiruppathi, T Naqvi, Y Wu… - Proceedings of the …, 2004 - National Acad Sciences
... Rensselaer, NY). Peptides were synthesized as C-terminal amide (
bioWORLD,
Dublin,
OH). Their purity and amino acid sequence were determined by HPLC and MS, respectively. Peptides used in this study were 98% pure. BSA
... Cited by 53 -
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[HTML] from plantphysiol.orgL Hopkins, S Parmar, A Blaszczyk, H Hesse… - Plant …, 2005 - Am Soc Plant Biol
... Antibody Production and Western Blotting. A rabbit antisera against a 20mer oligopeptide situated near to the N-terminal region of StST1 (NMATDISRVASSRRHSENGL) coupled to keyhole limpet hemocyanin was prepared (
Bioworld,
Dublin,
OH).
... Cited by 41 -
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All 10 versions
RC Gillis, BJ Daley, BL Enderson… - The Journal of …, 2003 - journals.lww.com
... Louis, MO), 100 mmol/L Tris, pH 8.0, and 0.8% (w/v) sodium lauryl sarcosine (
Bioworld,
Dublin,
OH). RNase/T1 cocktail mix (10 μL 1 mg/mL; Ambion, Austin, TX) was added and mixed well by flicking the tip of the tube. This was incubated at 37°C for 75 minutes.
... Cited by 5 -
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[CITATION] Potential role for Id myogenic repressors in apoptosis and attenuation of hypertrophy in muscles of aged rats
Free from PublisherSE Alway, H Degens… - American Journal of …, 2002 - Am Physiological Soc
Cited by 75 -
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[PDF] from biomedcentral.comR Alvarez… - Virology Journal, 2005 - biomedcentral.com
... Rabbit pre- immune sera were used as negative controls for rabbit assays. SDS-PAGE and Western blot assay Protein concentration of the supernatant fraction from infected cells was measured for protein concentration by Bradford's reagent (
Bioworld,
Dublin,
OH) at 595 nm.
... Cited by 3 -
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[HTML] from jlr.orgGM Hatch, AJ Smith, FY Xu, AM Hall… - Journal of lipid research, 2002 - ASBMB
... Once established, several lines were analyzed for FATP1 expression using a commercially generated (
bioWORLD,
Dublin OH) affinity-purified polyclonal anti-peptide antibody made in rabbits and directed toward murine FATP1 amino acid sequences 192–215.
... Cited by 38 -
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A Dibas, G Prasanna… - Journal of Ocular Pharmacology …, 2005 - liebertonline.com
... (St. Louis, MO). Big ET-1 (16–37) was synthesized by
BioWorld Labs (
Dublin,
OH). PVDF (Polyvinylidene difluo- ride) membranes were purchased from Millipore (Billerica, MA). Anticathepsin antibodies were purchased from Santa Cruz (Santa Cruz, CA).
... Cited by 5 -
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[HTML] from cell.comTM Grieco, JD Malhotra, C Chen, LL Isom… - Neuron, 2005 - Elsevier
Cited by 69 -
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[HTML] from genetics.orgJ Subramanian, S Vijayakumar, AE Tomkinson… - Genetics, 2005 - Genetics Soc America
... TNR instability assay, plasmids containing TNR sequences (pBL69, pBL70, pBL144, and pBL145; described later) were digested with Bsu36I (for targeting to the LYS2 locus) and used for the transformation (Fast Track Yeast Transformation kit;
Bioworld,
Dublin,
OH) of BY4741
... Cited by 15 -
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[PDF] from dtic.milWL Rust… - 2002 - oai.dtic.mil Page 1. 夕 AD Award Number: DAMD17-00-1-0363 TITLE: Elucidation of Pertussis Toxin-Sensitive Signaling in Human Breast Cancer Cells Migration PRINCIPAL INVESTIGATOR: William L. Rust, Ph.D CONTRACTING ORGANIZATION
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SP Colgan… - US Patent App. 10/910,802, 2004 - Google Patents Page 1. US 20050169914A1 (19) United States (12) Patent Application Publication (io> Pub. NO.: US 2005/0169914 Al Colgan et al. (43) Pub. Date: Aug. 4,2005 (54) NOVEL FUNCTIONS FOR DECAY ACCELERATING FACTOR
... All 4 versions
[PDF] from ttu.eduMK Lackey - 2003 - etd.lib.ttu.edu Page 1. CHARACTERIZATION OF AN ORF IN A NOVEL ORIENTATION IN Escherichia coli by MELINDA K. LACKEY, BS A THESIS IN MEDICAL MICROBIOLOGY Submitted to the Graduate Faculty of Texas Tech University
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MP Zimmerman… - US Patent App. 09/878,775, 2001 - Google Patents
... See, eg, Thornberry et al., 356 Nature 755; Goll et al., 74 Biochimi 225 (1992), and
Bioworld Today, Vol. 3, No. 67, p. 1, Apr. 3, 1992.
... [0011] Similarly, reversible inhibitors to serine proteases also exist in their hydrated form: HO
OH peptide C CF3 [0012] and therefore become
... Related articles -
All 7 versions
[PDF] from nitisurgical.comH Johnson - BBI NEWSLETTER, 2005 - nitisurgical.com
... Now available online: Go to www.ahcpub.com/online.html for details © Copyright 2005 by Thomson
BioWorld ®. Unauthorized photocopying or distribution is strictly prohibited by law.
... bbi1205a.qxd 11/18/2005 5:12 PM Page 1 Page 2. © Copyright 2005 by Thomson
BioWorld ®.
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[HTML] from gwdg.deM Grzmil - 2003 - webdoc.sub.gwdg.de Page 1. Isolation and functional analysis of differentially expressed genes in human prostate cancer Dissertation zur Erlangung des Doktorgrades der Mathematisch-Naturwissenschaftlichen Fakultäten der Georg-August-Universität zu Göttingen vorgelegt von Michal Grzmil
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G Giannachi… - 2005 - books.google.com Page 1. GABRIELLA GIANNACHI & NIGEL STEWART (EDS) EXPLORATIONS\ n ECOLOGY^\ ND THE ARTS PETER LANG Page 2. Page 3. Performing Nature This On© 9FAN-Q57-9L1H Page 4. Page 5. Performing Nature EXPLORATIONS
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