[HTML] from nih.govP Labhart - Molecular and cellular biology, 1999 - Am Soc Microbiol
... The 46.9-kDa protein (his-DNPK) was purified on nickel-nitrilotriacetic acid-agarose under denaturing conditions and further purified by preparative SDS-12% polyacrylamide gel electrophoresis for the production of polyclonal antibodies in rabbits (
BioWorld,
Dublin,
Ohio).
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[HTML] from nih.govPH King - Nucleic Acids Research, 2000 - Oxford Univ Press
... The probes were quantitated using a commercial assay (
BioWorld,
Dublin,
OH). Preparation of recombinant proteins and ELISA plates. HuC and HuD were expressed as fusion proteins with a 6-histidine tag as previously described (15).
... Cited by 33 -
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[HTML] from pnas.orgP Van Rhijn, Y Fang, S Galili… - Proceedings of the …, 1997 - National Acad Sciences
... of the hybridization period, the membrane was washed once in 1× SSC/0.5% SDS for 10 min at room temperature, and then twice in 0.1× SSC/0.5% SDS at 55°C. The filters were then exposed to x-ray film (Fuji photo film, Japan or
Bioworld,
Dublin,
OH) at −70°C for 1–10 days.
... Cited by 156 -
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JL Cook, V Marcheselli, J Alam, PL Deininger… - Brain Research …, 1998 - Elsevier
... Supported nitrocellulose membranes were from Schleicher and Schuell (Keene, NH, USA). Bio-Spin 30 columns were from Bio-Rad Laboratories (Hercules, CA, USA). Kapak tubular roll stock for heat seal bags was from
BioWorld (
Dublin,
OH, USA). 3.3. Special equipment.
... Cited by 3 -
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[HTML] from plantphysiol.orgPC Sehnke, R Henry, K Cline… - Plant physiology, 2000 - Am Soc Plant Biol
... Mab) containing ascites fluid (Lu et al., 1992 ) (1/3,000) or rabbit polyclonal anti-GF14 sera (1/10,000) produced by
Bioworld (
Dublin,
OH) using conjugated peptides derived from the different At GF14 isoform cDNA sequences (PC Sehnke and RJ Ferl, unpublished data).
... Cited by 71 -
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[HTML] from cjb.netF Xu… - Journal of Neuroscience, 1999 - neuro.cjb.net
... PLC293ct was purified from lysed bacteria, using Ni-NTA agarose per the supplier's instructions (protocol 14; Qiagen, Valencia, CA), and was used for the commercial production of a rabbit antiserum named P293 (
BioWorld,
Dublin,
OH).
... Cited by 24 -
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F Xu, SC Bose… - The Journal of …, 1999 - interscience.wiley.com
... G293ct and the G12 peptide-ovalbumin complex were used for commercial production of rabbit antisera G293 and G12, respectively (
BioWorld,
Dublin,
OH). When G293ct was used for functional studies, the amino acids encoded by the vector were removed with thrombin.
... Cited by 8 -
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RL Widom, I Culic, JY Lee… - Gene, 1997 - Elsevier
... The fusion protein was expressed in E. coli strain BL21 and purified using glutathione-sepharose as directed by the manufacturer (Pharmacia). Protein purified from a preparative SDS–PAGE gel was used to raise polyclonal rabbit antibodies (
BioWorld,
Dublin,
OH).
... Cited by 38 -
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BJ Dolnick, K Lu, MB Yin… - Advances in Enzyme Regulation, 1997 - Elsevier
... MA). Amiigase (thermally stable DNA ligase, EC 6.5.1.1) and gelase (agarase, EC 3.2.1.81) were obtained from Epicentre (Madison, WI). IsopropylthioDgalactoside (IPTG) was from
BioWorld (
Dublin,
OH). Oli godeoxyribonucleotides
... Cited by 11 -
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[HTML] from ukpmc.ac.ukPH King - Nucleic Acids Research, 2000 - ukpmc.ac.uk
... The probes were quantitated using a commercial assay (
BioWorld,
Dublin,
OH). Preparation of recombinant proteins and ELISA plates. HuC and HuD were expressed as fusion proteins with a 6-histidine tag as previously described (15).
... Related articles
B Honoré - Biochimica et Biophysica Acta (BBA)-Gene Structure …, 2000 - Elsevier
... peroxidase-labelled streptavidin and visualisation using chemiluminescence. The analysis was performed as a service by
Bio-
World Laboratory Essentials (
Dublin,
OH, USA). 2.7. Production of antibodies against hnRNP 2H9 proteins.
... Cited by 14 -
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[HTML] from plantphysiol.orgBJ Laughner, PC Sehnke… - Plant physiology, 1998 - Am Soc Plant Biol
... Once again, fractions containing NRX were pooled and their purity was estimated at greater than 95% by Coomassie blue staining. These NRX fractions were used for the production of polyclonal rabbit antibodies (
Bioworld,
Dublin,
OH).
... Cited by 27 -
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[HTML] from aacrjournals.orgR Thomas, LD True, JA Bassuk, PH Lange… - Clinical Cancer …, 2000 - AACR
... For paraffin-embedded sections, tissues samples were fixed in either formaldehyde (10% v/v; Mallinckrodt Baker Inc., Paris, KY) or in Histochoice (
Bio World Laboratories,
Dublin,
OH) before embedding in paraffin. Metastatic
... Cited by 91 -
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[HTML] from ukpmc.ac.ukP van Rhijn, Y Fang, S Galili, O Shaul… - Proceedings of the …, 1997 - ukpmc.ac.uk
... of the hybridization period, the membrane was washed once in 1× SSC/0.5% SDS for 10 min at room temperature, and then twice in 0.1× SSC/0.5% SDS at 55°C. The filters were then exposed to x-ray film (Fuji photo film, Japan or
Bioworld,
Dublin,
OH) at −70°C for 1–10 days.
... Related articles
RP Tuori, TJ Wolpert… - Molecular Plant-Microbe …, 2000 - Am Phytopath Society
... Sequence determination was performed at the Biotechnology Lab at the Institute for Molecular Biology, University of Ore- gon. Antiserum against refolded, purified NC-FP was raised at
Bioworld (
Dublin,
OH). Determination of the N terminus of the mature Ptr ToxA protein.
Cited by 23 -
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[PDF] from dtic.milNG Bazan… - 1998 - oai.dtic.mil
... 16 1С Somatostatin 5 50 «I* On In I* TIM Post-Inlurr 38 Thymome kimm 5 90 «I in M Tim post-miury *2 S so S a
OH lit Iwk Tiw Post-Injury I 1S S | «о Î2 0П in ink Tia» Poet-Injury IE PU> гоо ; íso 5 ã S loo - 1 g 50 S on in M TiM Poat-Injgry 5 so Il u \\ e an in ink rìw Post-Ini игу 5 гоо
... All 4 versions
C Barker… - 2000 - books.google.com Page 1. CAMBRIDGE UNIVERSITY PRESS Page 2. Page 3. NEW^ THEATRE QUARTERLY RUSSELL JACKSON 107 BAZ KERSHAW 122 BARNABY KING 131 CLAIRE COCHRANE 137 MARTIN ROHMER 148 CAROLEE
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