Bio LC Affinity Media

BlackPearl®-A (Protein A) |Cat# 20161000-1 |Size 1mL EA | $ 87.73

Black Pearl-A™ (PROTEIN A),

An affinity matrix ideal for isolation and purification of immunoglobins on a small scale. Black Pearl-A™ magnetic beads consist of recombinant Protein A covalently coupled to Black Pearl™ Magnetic Beads. Protein A has a high affinity for subclasses of IgG from a variety of species, including human, rabbit, mouse, and sheep. Coupled through a stable and leak resistant linkage over a wide pH range, this allows immunomagnetic purifications of IgG\'s from ascites, serum, or cell culture supernatants. The matrix can be regenerated up to 6 times with minimal loss of binding capacity. It can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. Specific antibodies can be covalently cross-linked to the Black Pearl-A™ surface to create a reusable immunoprecipitation bead, thus avoiding the co-elution of antibody with the target antigen.

Specifications:

Matrix: Cross-linked Agarose 6%

Particle Size (range): 50-125 µm

pH stability: 4-9

Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-A (Protein A) |Cat# 20161000-2 |Size 5mL EA | $ 266.61

Black Pearl-A™ (PROTEIN A),

An affinity matrix ideal for isolation and purification of immunoglobins on a small scale. Black Pearl-A™ magnetic beads consist of recombinant Protein A covalently coupled to Black Pearl™ Magnetic Beads. Protein A has a high affinity for subclasses of IgG from a variety of species, including human, rabbit, mouse, and sheep. Coupled through a stable and leak resistant linkage over a wide pH range, this allows immunomagnetic purifications of IgG\'s from ascites, serum, or cell culture supernatants. The matrix can be regenerated up to 6 times with minimal loss of binding capacity. It can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. Specific antibodies can be covalently cross-linked to the Black Pearl-A™ surface to create a reusable immunoprecipitation bead, thus avoiding the co-elution of antibody with the target antigen.

Specifications:

Matrix: Cross-linked Agarose 6%

Particle Size (range): 50-125 µm

pH stability: 4-9

Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-C (Calmodulin) |Cat# 20161002-1 |Size 1mL EA | $ 98.5

Black Pearl-C™ (CALMODULIN)

A Magnetic Bead Affinity Matrix for immobilized Calmodulin. For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.

Specifications:

Supplied as suspension in 0.5 M NaCl.

Matrix: Cross-linked Agarose 6%

Particle Size (range): 25-75 µm

pH stability: 4-9

Storage: 2-8 °C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-C (Calmodulin) |Cat# 20161002-2 |Size 5mL EA | $ 227.6

Black Pearl-C™ (CALMODULIN)

A Magnetic Bead Affinity Matrix for immobilized Calmodulin. For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.

Specifications:

Supplied as suspension in 0.5 M NaCl.

Matrix: Cross-linked Agarose 6%

Particle Size (range): 25-75 µm

pH stability: 4-9

Storage: 2-8 °C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-C (Calmodulin) |Cat# 20161002-3 |Size 10mL EA | $ 353.68

Black Pearl-C™ (CALMODULIN)

A Magnetic Bead Affinity Matrix for immobilized Calmodulin. For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.

Specifications:

Supplied as suspension in 0.5 M NaCl.

Matrix: Cross-linked Agarose 6%

Particle Size (range): 25-75 µm

pH stability: 4-9

Storage: 2-8 °C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-G (Protein G) |Cat# 20161003-1 |Size 1mL EA | $ 98.5

Black Pearl-G™ (PROTEIN G)

Magnetic beads are prepared by covalent immobilization of native protein G (from Streptococcus sp.) to Black Pearl™ Magnetic Beads by CNBr activation coupling chemistry.

Protein G shows strong binding affinity to Fc region of several classes of mammalian immunoglobulins, Black Pearl-G™ has been used as a reliable tool to purify monoclonal and polyclonal IgGs from ascites, serum and cell culture supernatants. It is an ideal choice for affinity purification and/or fractionation of IgG subclasses which binds poorly to protein A such as bovine, goat, rat, and sheep IgG.

These products are supplied as suspension in a buffer containing 100 mM Tris Cl, pH 8.0, 500 mM NaCl, 0.05 % NaN3, and 0.02 % thimerosal.

Specifications:

Ligand: Native protein G from Streptococcus sp.

Ligand coupling method: CNBr activation

Biding capacity: Approximately 20 mg human IgG/ml drained gel

Matrix: Agarose, 4%

Particle size (range): 50-125 µm

pH stability: 3-9 (for use), 2-10 (for cleaning)

Storage: 2-8 °C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-G (Protein G) |Cat# 20161003-2 |Size 5mL EA | $ 398.7

Black Pearl-G™ (PROTEIN G)

Magnetic beads are prepared by covalent immobilization of native protein G (from Streptococcus sp.) to Black Pearl™ Magnetic Beads by CNBr activation coupling chemistry.

Protein G shows strong binding affinity to Fc region of several classes of mammalian immunoglobulins, Black Pearl-G™ has been used as a reliable tool to purify monoclonal and polyclonal IgGs from ascites, serum and cell culture supernatants. It is an ideal choice for affinity purification and/or fractionation of IgG subclasses which binds poorly to protein A such as bovine, goat, rat, and sheep IgG.

These products are supplied as suspension in a buffer containing 100 mM Tris Cl, pH 8.0, 500 mM NaCl, 0.05 % NaN3, and 0.02 % thimerosal.

Specifications:

Ligand: Native protein G from Streptococcus sp.

Ligand coupling method: CNBr activation

Biding capacity: Approximately 20 mg human IgG/ml drained gel

Matrix: Agarose, 4%

Particle size (range): 50-125 µm

pH stability: 3-9 (for use), 2-10 (for cleaning)

Storage: 2-8 °C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-L (Lectin) |Cat# 20161005-1 |Size 1mL EA | $ 92.5

Black Pearl-L™ (Wheat Germ Lectin)

Wheat germ lectin (Isolated from Triticum vulgaris) conjugated to Black Pearl™ Magnetic Beads.

Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing.

Specifications:

• Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine, Chitin Hydrolysate or 100 mM acetic acid
• Binding: 2 mg lectin/ml gel
• Particle size: 50-125 µm
• Needs Neodyne Magnets for attracting beads.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®-L (Lectin) |Cat# 20161005-2 |Size 5mL EA | $ 298.5

Black Pearl-L™ (Wheat Germ Lectin)

Wheat germ lectin (Isolated from Triticum vulgaris) conjugated to Black Pearl™ Magnetic Beads.

Wheat germ agglutinin is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing.

Specifications:

• Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine, Chitin Hydrolysate or 100 mM acetic acid
• Binding: 2 mg lectin/ml gel
• Particle size: 50-125 µm
• Needs Neodyne Magnets for attracting beads.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl® magnetic beads |Cat# 20161006-1 |Size 5mL EA | $ 77.61

Black Pearl Magnetic Beads

Utilized in the magnetic separation of cells, organelles, proteins, immunoglobins, nucleic acids, and many other types of molecules in biological and non-biological systems. BlackPearl beads are easier and more cost effective than traditional column affinity chromatography beads. They are made with biotechnology grade agarose and magnetic particles. Magnetic beads substantially shorten each step of purification because of their easy magnetic retrieval. Exposure to sample, washing of un-bound samples and washing the target components free of the ligand on the bead are all made easier and quicker. Magnetic bioseparation can also avoid unnecessary dilution introduced by typical column elution procedures.

Specifications:

Matrix: Agarose, 6% cross-linked beads

Particle Size (range): 50-125µm

pH stability: 4-9

Storage: 2-8 °C

Supplied as slurry in 20% ethanol

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl® magnetic beads |Cat# 20161006-2 |Size 25mL EA | $ 213.61

Black Pearl Magnetic Beads

Utilized in the magnetic separation of cells, organelles, proteins, immunoglobins, nucleic acids, and many other types of molecules in biological and non-biological systems. BlackPearl beads are easier and more cost effective than traditional column affinity chromatography beads. They are made with biotechnology grade agarose and magnetic particles. Magnetic beads substantially shorten each step of purification because of their easy magnetic retrieval. Exposure to sample, washing of un-bound samples and washing the target components free of the ligand on the bead are all made easier and quicker. Magnetic bioseparation can also avoid unnecessary dilution introduced by typical column elution procedures.

Specifications:

Matrix: Agarose, 6% cross-linked beads

Particle Size (range): 50-125µm

pH stability: 4-9

Storage: 2-8 °C

Supplied as slurry in 20% ethanol

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl® magnetic beads |Cat# 20161006-3 |Size 50mL EA | $ 390.99

Black Pearl Magnetic Beads

Utilized in the magnetic separation of cells, organelles, proteins, immunoglobins, nucleic acids, and many other types of molecules in biological and non-biological systems. BlackPearl beads are easier and more cost effective than traditional column affinity chromatography beads. They are made with biotechnology grade agarose and magnetic particles. Magnetic beads substantially shorten each step of purification because of their easy magnetic retrieval. Exposure to sample, washing of un-bound samples and washing the target components free of the ligand on the bead are all made easier and quicker. Magnetic bioseparation can also avoid unnecessary dilution introduced by typical column elution procedures.

Specifications:

Matrix: Agarose, 6% cross-linked beads

Particle Size (range): 50-125µm

pH stability: 4-9

Storage: 2-8 °C

Supplied as slurry in 20% ethanol

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®(magenitic beads)-GSH (Glutathione) |Cat# 20161007-1 |Size 1mL EA | $ 59.7

Blackpearl^™-GSH are paramagnetic nano particles embedded in cross-linked Separopore® 4B. This affinity matrix is designed for use in automated as well as small scale affinity purification of  proteins with glutathione binding sequences eg., native Glutathione-S-transferase (GST), Glutathione peroxidase and Glyoxalase I. The reduced glutathione ligand is covalently attached to Blackpearl magnetic beads with spacer arm of 10 atoms.

Technical Specifications

• Matrix: 4% agarose beads impregnated with paramagnetic iron.
• Ligand: Glutathione (GSH).
• Ligand coupling: Epoxy activation.
• Particle size: 30-85µm.
• Binding capacity: >5mg GST/ml drained suspension.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

BlackPearl®(magenitic beads)-GSH (Glutathione) |Cat# 20161007-2 |Size 5mL EA | $ 165

Blackpearl^™-GSH are paramagnetic nano particles embedded in cross-linked Separopore® 4B. This affinity matrix is designed for use in automated as well as small scale affinity purification of  proteins with glutathione binding sequences eg., native Glutathione-S-transferase (GST), Glutathione peroxidase and Glyoxalase I. The reduced glutathione ligand is covalently attached to Blackpearl magnetic beads with spacer arm of 10 atoms.

Technical Specifications

• Matrix: 4% agarose beads impregnated with paramagnetic iron.
• Ligand: Glutathione (GSH).
• Ligand coupling: Epoxy activation.
• Particle size: 30-85µm.
• Binding capacity: >5mg GST/ml drained suspension.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Calmodulin Separopore® 4B |Cat# 20181001-1 |Size 1mL EA | $ 87.5

Calmodulin Separopore® 4B

• Affinity matrix with immobilized bovine calmodulin.
• For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.
• Supplied as suspension in 10% ethanol.

Technical Specifications

• Ligand: calmodulin
• Ligand coupling method: CNBr activation
• Ligand density: approximately 1mg calmodulin/ml resin
• Matrix: agarose, 4%
• Exclusion limit (Mr): approximately 2 x 10⁷
• Particle size (range): 45-165µm
• pH stability: 4-9
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Calmodulin Separopore® 4B |Cat# 20181001-2 |Size 5mL EA | $ 169.5

Calmodulin Separopore® 4B

• Affinity matrix with immobilized bovine calmodulin.
• For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.
• Supplied as suspension in 10% ethanol.

Technical Specifications

• Ligand: calmodulin
• Ligand coupling method: CNBr activation
• Ligand density: approximately 1mg calmodulin/ml resin
• Matrix: agarose, 4%
• Exclusion limit (Mr): approximately 2 x 10⁷
• Particle size (range): 45-165µm
• pH stability: 4-9
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Calmodulin Separopore® 4B |Cat# 20181001-3 |Size 10mL EA | $ 298.7

Calmodulin Separopore® 4B

• Affinity matrix with immobilized bovine calmodulin.
• For affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.
• Supplied as suspension in 10% ethanol.

Technical Specifications

• Ligand: calmodulin
• Ligand coupling method: CNBr activation
• Ligand density: approximately 1mg calmodulin/ml resin
• Matrix: agarose, 4%
• Exclusion limit (Mr): approximately 2 x 10⁷
• Particle size (range): 45-165µm
• pH stability: 4-9
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CDI-activated Separopore® 4B |Cat# 20181002-1 |Size 5mL EA | $ 52.45

CDI-activated Separopore® 4B

 

• Pre-activated medium for immobilization of ligands containing primary amines.
• N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• Supplied as gel suspension in acetone.

Technical Specifications

• Group to be coupled: -NH2
• Chemical linkage of ligand: stable amide carbamate linkage
• Matrix: cross-linked agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 11 (ligand dependent)
• Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CDI-activated Separopore® 4B |Cat# 20181002-2 |Size 25mL EA | $ 150.54

CDI-activated Separopore® 4B

 

• Pre-activated medium for immobilization of ligands containing primary amines.
• N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• Supplied as gel suspension in acetone.

Technical Specifications

• Group to be coupled: -NH2
• Chemical linkage of ligand: stable amide carbamate linkage
• Matrix: cross-linked agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 11 (ligand dependent)
• Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CDI-activated Separopore® 4B |Cat# 20181002-3 |Size 100mL EA | $ 396.4

CDI-activated Separopore® 4B

 

• Pre-activated medium for immobilization of ligands containing primary amines.
• N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• Supplied as gel suspension in acetone.

Technical Specifications

• Group to be coupled: -NH2
• Chemical linkage of ligand: stable amide carbamate linkage
• Matrix: cross-linked agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 11 (ligand dependent)
• Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 4B |Cat# 20181003-1 |Size 1g EA | $ 64.32

CNBR-Activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 4% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 4B |Cat# 20181003-2 |Size 5g EA | $ 178.84

CNBR-Activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 4% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 4B |Cat# 20181003-3 |Size 10g EA | $ 280.08

CNBR-Activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 4% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

DEAE Separopore® CL-6B |Cat# 20181004-1 |Size 50mL EA | $ 103.98

BioWorld\'s DEAE Separopore® was prepared by covalent attachment of diethylaminoethyl to Agarose CL-6B. DEAE Separopore® is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. Diethylaminoethyl remains charges and maintains high capacities over a wide working range of pH 3-9. Excellent matrix for analytical and preparative fractionation of proteins. DEAE Separopore® is supplied as pre-swollen gel in 20% ethanol.

Specifications:

Ligand: diethylaminoethyl

Ligand coupling method: CNBr activation

Max flow rate: 150 cm/h

Binding capacity: 50-170mg/ml

Matrix: Agarose, 6% cross-linked

Bead size range: 50-175 um

pH stability: 3-9

Storage: 2-8 C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

DEAE Separopore® CL-6B |Cat# 20181004-2 |Size 100mL EA | $ 177.72

BioWorld\'s DEAE Separopore® was prepared by covalent attachment of diethylaminoethyl to Agarose CL-6B. DEAE Separopore® is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. Diethylaminoethyl remains charges and maintains high capacities over a wide working range of pH 3-9. Excellent matrix for analytical and preparative fractionation of proteins. DEAE Separopore® is supplied as pre-swollen gel in 20% ethanol.

Specifications:

Ligand: diethylaminoethyl

Ligand coupling method: CNBr activation

Max flow rate: 150 cm/h

Binding capacity: 50-170mg/ml

Matrix: Agarose, 6% cross-linked

Bead size range: 50-175 um

pH stability: 3-9

Storage: 2-8 C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

DEAE Separopore® CL-6B |Cat# 20181004-3 |Size 500mL EA | $ 482.44

BioWorld\'s DEAE Separopore® was prepared by covalent attachment of diethylaminoethyl to Agarose CL-6B. DEAE Separopore® is a weak anion exchanger with excellent flow capacities and a high capacity for binding and resolution of proteins of all pI values. Diethylaminoethyl remains charges and maintains high capacities over a wide working range of pH 3-9. Excellent matrix for analytical and preparative fractionation of proteins. DEAE Separopore® is supplied as pre-swollen gel in 20% ethanol.

Specifications:

Ligand: diethylaminoethyl

Ligand coupling method: CNBr activation

Max flow rate: 150 cm/h

Binding capacity: 50-170mg/ml

Matrix: Agarose, 6% cross-linked

Bead size range: 50-175 um

pH stability: 3-9

Storage: 2-8 C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Dextran Separopore® |Cat# 20181005-1 |Size 25mL EA | $ 102.44

Dextran coupled to a high strength Separopore® 4B matrix. Coupling Dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules.

Dextran Separopore® |Cat# 20181005-2 |Size 50mL EA | $ 181.75

Dextran coupled to a high strength Separopore® 4B matrix. Coupling Dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules.

Dextran Separopore® |Cat# 20181005-3 |Size 100mL EA | $ 308.39

Dextran coupled to a high strength Separopore® 4B matrix. Coupling Dextran to this material expands the surface area of the Separopore® beads allowing for further binding of macromolecules.

E. coli protein Separopore® |Cat# 20181006-1 |Size 1mL EA | $ 65.23

E.coli Protein Separopore®

Bio-World offers our immobilized E. coli proteins on a solid bio-affinity support. Our Immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of Immobilized E. coli lysate thus functions as a \"reverse affinity matrix\". The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.

E.coli proteins were extracted from three strains of E.coli and coupled to CNBr activated Separopore® 4B.

Anti-coli protein antibodies can be removed by solid phase immunoaffinity chromatography over the anti-coli Separopore® column.

Supplied as 1, 5, or 10ml sizes settled gel in PBS and sodium azide.

Do not freeze.

Store at 4°C in the presence of sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

E. coli protein Separopore® |Cat# 20181006-2 |Size 5mL EA | $ 196.27

E.coli Protein Separopore®

Bio-World offers our immobilized E. coli proteins on a solid bio-affinity support. Our Immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of Immobilized E. coli lysate thus functions as a \"reverse affinity matrix\". The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.

E.coli proteins were extracted from three strains of E.coli and coupled to CNBr activated Separopore® 4B.

Anti-coli protein antibodies can be removed by solid phase immunoaffinity chromatography over the anti-coli Separopore® column.

Supplied as 1, 5, or 10ml sizes settled gel in PBS and sodium azide.

Do not freeze.

Store at 4°C in the presence of sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

E. coli protein Separopore® |Cat# 20181006-3 |Size 10mL EA | $ 278.95

E.coli Protein Separopore®

Bio-World offers our immobilized E. coli proteins on a solid bio-affinity support. Our Immobilized E. coli lysate consists of partially purified proteins from E. coli strains commonly used for protein expression. The coupling chemistry is leak-resistant and provides a matrix with very low nonspecific binding. A column of Immobilized E. coli lysate thus functions as a \"reverse affinity matrix\". The contaminating E. coli antibodies are absorbed onto the matrix and the purified antisera passes through for further use.

E.coli proteins were extracted from three strains of E.coli and coupled to CNBr activated Separopore® 4B.

Anti-coli protein antibodies can be removed by solid phase immunoaffinity chromatography over the anti-coli Separopore® column.

Supplied as 1, 5, or 10ml sizes settled gel in PBS and sodium azide.

Do not freeze.

Store at 4°C in the presence of sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

EAH Separopore® 4B |Cat# 20181007-1 |Size 1mL EA | $ 52.52

BioWorld\'s EAH-Separopore® was prepared by a covalent coupling of 1,6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), EAH-Separopore® is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Specifications

• Matrix: Agarose, 4% beads.
• Group to be coupled: -COOH, -CHO.
• Chemical linkage of ligand: stable amide linkage.
• Particle size (range):90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling condition: pH 4.5-6, 4-25°C.
• Storage 20% ethanol containing 0.9% NaCl. 
• Substitution 7-11 µmol amino/ml medium.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

EAH Separopore® 4B |Cat# 20181007-2 |Size 15mL EA | $ 110.22

BioWorld\'s EAH-Separopore® was prepared by a covalent coupling of 1,6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), EAH-Separopore® is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Specifications

• Matrix: Agarose, 4% beads.
• Group to be coupled: -COOH, -CHO.
• Chemical linkage of ligand: stable amide linkage.
• Particle size (range):90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling condition: pH 4.5-6, 4-25°C.
• Storage 20% ethanol containing 0.9% NaCl. 
• Substitution 7-11 µmol amino/ml medium.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

EAH Separopore® 4B |Cat# 20181007-3 |Size 25mL EA | $ 223.49

BioWorld\'s EAH-Separopore® was prepared by a covalent coupling of 1,6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), EAH-Separopore® is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Specifications

• Matrix: Agarose, 4% beads.
• Group to be coupled: -COOH, -CHO.
• Chemical linkage of ligand: stable amide linkage.
• Particle size (range):90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling condition: pH 4.5-6, 4-25°C.
• Storage 20% ethanol containing 0.9% NaCl. 
• Substitution 7-11 µmol amino/ml medium.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

ECH Separopore® 4B |Cat# 20181008-1 |Size 5mL EA | $ 38.7

ECH Separopore® 4B

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are to be coupled in a single-step procedure in the presence of a coupling reagent (not provided), a water-soluble carbodiimide, such as N-ethyl-N\'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.
• Supplied as pre-swelled gel suspension in buffered saline.

Technical Specifications

• Group to be coupled: -NH2.
• Chemical linkage of ligand: stable amide linkage.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling conditions: pH 4.5-6, 4-25ºC, 2-24 h.
• storage: 2-8ºC.
• Substitution 12-16 µmol carboxyl/ml medium. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ECH Separopore® 4B |Cat# 20181008-2 |Size 15mL EA | $ 52.3

ECH Separopore® 4B

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are to be coupled in a single-step procedure in the presence of a coupling reagent (not provided), a water-soluble carbodiimide, such as N-ethyl-N\'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.
• Supplied as pre-swelled gel suspension in buffered saline.

Technical Specifications

• Group to be coupled: -NH2.
• Chemical linkage of ligand: stable amide linkage.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling conditions: pH 4.5-6, 4-25ºC, 2-24 h.
• storage: 2-8ºC.
• Substitution 12-16 µmol carboxyl/ml medium. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ECH Separopore® 4B |Cat# 20181008-3 |Size 25mL EA | $ 168.37

ECH Separopore® 4B

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are to be coupled in a single-step procedure in the presence of a coupling reagent (not provided), a water-soluble carbodiimide, such as N-ethyl-N\'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.
• Supplied as pre-swelled gel suspension in buffered saline.

Technical Specifications

• Group to be coupled: -NH2.
• Chemical linkage of ligand: stable amide linkage.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling conditions: pH 4.5-6, 4-25ºC, 2-24 h.
• storage: 2-8ºC.
• Substitution 12-16 µmol carboxyl/ml medium. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ECH Separopore® 4B |Cat# 20181008-4 |Size 50mL EA | $ 277.83

ECH Separopore® 4B

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are to be coupled in a single-step procedure in the presence of a coupling reagent (not provided), a water-soluble carbodiimide, such as N-ethyl-N\'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.
• Supplied as pre-swelled gel suspension in buffered saline.

Technical Specifications

• Group to be coupled: -NH2.
• Chemical linkage of ligand: stable amide linkage.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-amino caproic acid, 10 atom.
• Coupling conditions: pH 4.5-6, 4-25ºC, 2-24 h.
• storage: 2-8ºC.
• Substitution 12-16 µmol carboxyl/ml medium. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Epoxy-activated Separopore® 4B |Cat# 20181009-1 |Size 1g EA | $ 65.23

Epoxy-activated Separopore® 4B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder stabilised with lactose and dextran.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH2, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 4% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°
• Matrix active group Epoxy.
• Matrix spacer 12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether).
• Extent of labelling 20-40 μmol per mL.
• Attachment One epoxy group.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Epoxy-activated Separopore® 4B |Cat# 20181009-2 |Size 5g EA | $ 198.7

Epoxy-activated Separopore® 4B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder stabilised with lactose and dextran.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH2, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 4% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°
• Matrix active group Epoxy.
• Matrix spacer 12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether).
• Extent of labelling 20-40 μmol per mL.
• Attachment One epoxy group.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Epoxy-activated Separopore® 4B |Cat# 20181009-3 |Size 10g EA | $ 390.32

Epoxy-activated Separopore® 4B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder stabilised with lactose and dextran.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH2, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 4% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°
• Matrix active group Epoxy.
• Matrix spacer 12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether).
• Extent of labelling 20-40 μmol per mL.
• Attachment One epoxy group.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Epoxy-activated Separopore® 4B |Cat# 20181009-4 |Size 25g EA | $ 648.5

Epoxy-activated Separopore® 4B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 4B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder stabilised with lactose and dextran.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH2, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 4% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°
• Matrix active group Epoxy.
• Matrix spacer 12 atoms (when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether).
• Extent of labelling 20-40 μmol per mL.
• Attachment One epoxy group.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Fucose Separopore® |Cat# 20181010-1 |Size 10mL EA | $ 127

Affinity Matrix for fucose-binding glycoproteins. Fucose is linked to a fast flow Separopore®4B via a spacer arm to ensure effficient binding of this ligand to the protein.

Specifications:

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads, size (range): 50 -175 µm

Ligand: Fucose

Spacer arm: yes

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Fucose Separopore® |Cat# 20181010-2 |Size 25mL EA | $ 447.68

Affinity Matrix for fucose-binding glycoproteins. Fucose is linked to a fast flow Separopore®4B via a spacer arm to ensure effficient binding of this ligand to the protein.

Specifications:

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads, size (range): 50 -175 µm

Ligand: Fucose

Spacer arm: yes

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Galactose Separopore® |Cat# 20181011-1 |Size 10mL EA | $ 195

This product is composed of a Galactose molecule coupled with high strength bioagarose matrix.

Technical Specifications:

• Ligand: D-Galactose
•  Ligand coupling method: Covalent linkage
• Binding capacity: >20mg/ml
• Matrix: Agarose, Separopore®-4B
• Bead Size: 50- 160μm

 

 

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Gelatin Separopore® |Cat# 20181012-1 |Size 5mL EA | $ 33.39

Gelatin Separopore®

• Affinity matrix for the purification of fibronectin.
• Gelatin covalently coupled to Separopore® 4B by CNBr activation.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: Bovine gelatin.
• Ligand coupling method: CNBr activation.
• Ligand density: >5mg gelatin/ml.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10^7.
• Particle size (range)50-175µm.
• pH stability: 3-10.
• Storage 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Gelatin Separopore® |Cat# 20181012-2 |Size 10mL EA | $ 60.84

Gelatin Separopore®

• Affinity matrix for the purification of fibronectin.
• Gelatin covalently coupled to Separopore® 4B by CNBr activation.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: Bovine gelatin.
• Ligand coupling method: CNBr activation.
• Ligand density: >5mg gelatin/ml.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10^7.
• Particle size (range)50-175µm.
• pH stability: 3-10.
• Storage 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Gelatin Separopore® |Cat# 20181012-3 |Size 25mL EA | $ 110.51

Gelatin Separopore®

• Affinity matrix for the purification of fibronectin.
• Gelatin covalently coupled to Separopore® 4B by CNBr activation.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: Bovine gelatin.
• Ligand coupling method: CNBr activation.
• Ligand density: >5mg gelatin/ml.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10^7.
• Particle size (range)50-175µm.
• pH stability: 3-10.
• Storage 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Heparin Separopore® |Cat# 20181013-1 |Size Column EA | $ 55.67

Heparin Separopore®

• Affinity matrix with immobilized heparin (from porcine intestinal mucosa) by CNBr activation coupling method.
• For affinity purification of coagulation factors such as antithrombin III, DNA binding proteins, restriction endonucleases, lipases, growth factors, lipoproteins and steroid receptors.
• Supplied as suspension in 0.5M NaCl

Technical Specifications

• Ligand: porcine heparin
• Ligand coupling method: CNBr activation
• Ligand density: 5mg heparin/ml
• Binding capacity: approximately 2mg bovine antithrombin/ml gel
• Matrix: 4% Agarose beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 2-11 (ligand dependent)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Heparin Separopore® |Cat# 20181013-2 |Size 10mL EA | $ 107.5

Heparin Separopore®

• Affinity matrix with immobilized heparin (from porcine intestinal mucosa) by CNBr activation coupling method.
• For affinity purification of coagulation factors such as antithrombin III, DNA binding proteins, restriction endonucleases, lipases, growth factors, lipoproteins and steroid receptors.
• Supplied as suspension in 0.5M NaCl

Technical Specifications

• Ligand: porcine heparin
• Ligand coupling method: CNBr activation
• Ligand density: 5mg heparin/ml
• Binding capacity: approximately 2mg bovine antithrombin/ml gel
• Matrix: 4% Agarose beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 2-11 (ligand dependent)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Heparin Separopore® |Cat# 20181013-3 |Size 25mL EA | $ 189.5

Heparin Separopore®

• Affinity matrix with immobilized heparin (from porcine intestinal mucosa) by CNBr activation coupling method.
• For affinity purification of coagulation factors such as antithrombin III, DNA binding proteins, restriction endonucleases, lipases, growth factors, lipoproteins and steroid receptors.
• Supplied as suspension in 0.5M NaCl

Technical Specifications

• Ligand: porcine heparin
• Ligand coupling method: CNBr activation
• Ligand density: 5mg heparin/ml
• Binding capacity: approximately 2mg bovine antithrombin/ml gel
• Matrix: 4% Agarose beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 2-11 (ligand dependent)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Heparin Separopore® |Cat# 20181013-4 |Size 50mL EA | $ 335.98

Heparin Separopore®

• Affinity matrix with immobilized heparin (from porcine intestinal mucosa) by CNBr activation coupling method.
• For affinity purification of coagulation factors such as antithrombin III, DNA binding proteins, restriction endonucleases, lipases, growth factors, lipoproteins and steroid receptors.
• Supplied as suspension in 0.5M NaCl

Technical Specifications

• Ligand: porcine heparin
• Ligand coupling method: CNBr activation
• Ligand density: 5mg heparin/ml
• Binding capacity: approximately 2mg bovine antithrombin/ml gel
• Matrix: 4% Agarose beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• pH stability: 2-11 (ligand dependent)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

IgG Separopore® - fusion kit |Cat# 20181014-1 |Size 5mL KT | $ 166.47

IgG Separopor® - Protein A Conjugate binding kit

A complete kit to bind Protein A fusion proteins expressed in E. Coli or yeast. Includes binding & elution buffers.

IgG Separopore® 5ml

Binding Buffer

Elution Buffer

IgG (Rabbit) Separopore®4B |Cat# 20181015-1 |Size 1mL EA | $ 72.55

IgG-Separopore®

An affinity chromatography gel matrix consiting of rabbit IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

IgG (Rabbit) Separopore®4B |Cat# 20181015-2 |Size 5mL EA | $ 139.47

IgG-Separopore®

An affinity chromatography gel matrix consiting of rabbit IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

IgG (Rabbit) Separopore®4B |Cat# 20181015-3 |Size 10mL EA | $ 221.59

IgG-Separopore®

An affinity chromatography gel matrix consiting of rabbit IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

KLH Separopore® 4B |Cat# 20181016-1 |Size 1mL 2/PK | $ 67.14

KLH-Separopore® 4B

Affinity matrix with immobilized KLH for the purification of antibodies generated against a peptide-KLH conjugated antigens. It allows removal of antobodies against KLH portion of the antigen

Supplied as a suspension in PBS

Technical Specifications

• Ligand: Keyhole Limpet Hemocyanin (KLH)
• Ligand Coupling Method: CNBr Activation
• Ligand Density: Approximately 3mg KLH/ml drained gel
• Matrix: Agarose, 4% (beads)
• Exclusion Limit: Approximately 2 x 10⁷
• Particle Size (range): 50-175µm
• pH Stability; 4 - 9
• Storage: 2 - 8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

KLH Separopore® 4B |Cat# 20181016-2 |Size 10mL EA | $ 188.96

KLH-Separopore® 4B

Affinity matrix with immobilized KLH for the purification of antibodies generated against a peptide-KLH conjugated antigens. It allows removal of antobodies against KLH portion of the antigen

Supplied as a suspension in PBS

Technical Specifications

• Ligand: Keyhole Limpet Hemocyanin (KLH)
• Ligand Coupling Method: CNBr Activation
• Ligand Density: Approximately 3mg KLH/ml drained gel
• Matrix: Agarose, 4% (beads)
• Exclusion Limit: Approximately 2 x 10⁷
• Particle Size (range): 50-175µm
• pH Stability; 4 - 9
• Storage: 2 - 8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

KLH Separopore® 4B |Cat# 20181016-3 |Size 25mL EA | $ 335.2

KLH-Separopore® 4B

Affinity matrix with immobilized KLH for the purification of antibodies generated against a peptide-KLH conjugated antigens. It allows removal of antobodies against KLH portion of the antigen

Supplied as a suspension in PBS

Technical Specifications

• Ligand: Keyhole Limpet Hemocyanin (KLH)
• Ligand Coupling Method: CNBr Activation
• Ligand Density: Approximately 3mg KLH/ml drained gel
• Matrix: Agarose, 4% (beads)
• Exclusion Limit: Approximately 2 x 10⁷
• Particle Size (range): 50-175µm
• pH Stability; 4 - 9
• Storage: 2 - 8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lactose coupled Separopore® |Cat# 20181017-1 |Size 10mL EA | $ 197.58

Lactose Coupled Separopore® 4B

• Bio-World\'s Lactose Separopore® 4B was prepared by epoxy activation of Separopore.

• Lactose-Separopore® may be used to isolate lactose binding proteins, such as lectins.
• Please consult literature for suggested buffers and optimal pH for specific proteins.
• Lactose Separopore®4B is supplied as an aqueous suspension in 20 % ethanol.

Technical Specifications

• Ligand: Lactose.
• Ligand coupling method: Epoxy activation.
• Matrix:agarose, 4% beads.
• Binding Capacity: 20-25 mg/ml lectin/ml.
• Particle size (range): 50-160µm.
• Storage:2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lectin Separopore® 4B |Cat# 20181018-1 |Size 1mL EA | $ 85.08

Wheat Germ Lectin Separopore®4B

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies.

Specifications:

Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine or 100 mM acetic acid

Coupling Method: CNBr activation

pH stability: 4-9

Ligand Concentration: 5mg wheat germ lectin/mole of drained gel.

Supplied in 1% NaCl containing 0.04% sodium azide as a preservative

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lectin Separopore® 4B |Cat# 20181018-2 |Size 5mL EA | $ 179.7

Wheat Germ Lectin Separopore®4B

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies.

Specifications:

Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine or 100 mM acetic acid

Coupling Method: CNBr activation

pH stability: 4-9

Ligand Concentration: 5mg wheat germ lectin/mole of drained gel.

Supplied in 1% NaCl containing 0.04% sodium azide as a preservative

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lectin Separopore® 4B |Cat# 20181018-3 |Size 10mL EA | $ 298.36

Wheat Germ Lectin Separopore®4B

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies.

Specifications:

Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine or 100 mM acetic acid

Coupling Method: CNBr activation

pH stability: 4-9

Ligand Concentration: 5mg wheat germ lectin/mole of drained gel.

Supplied in 1% NaCl containing 0.04% sodium azide as a preservative

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lectin Separopore® 4B |Cat# 20181018-4 |Size 25mL EA | $ 487.56

Wheat Germ Lectin Separopore®4B

Wheat germ (Triticum vulgaris) agglutinin (WGA) is a 36,000 molecular weight protein consisting of two identical subunits. WGA contains a group of closely related isolectins, with an isoelectric point about pH 9. The receptor sugar for WGA is N-acetylglucosamine. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA). This lectin has proven useful for the purification of insulin receptors and for neuronal tracing studies.

Specifications:

Inhibiting/Eluting Sugar: 500mM N-acetylglucosamine or 100 mM acetic acid

Coupling Method: CNBr activation

pH stability: 4-9

Ligand Concentration: 5mg wheat germ lectin/mole of drained gel.

Supplied in 1% NaCl containing 0.04% sodium azide as a preservative

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Mannose Separopore® 4B |Cat# 20181019-1 |Size 10mL EA | $ 98.5

Mannose Separopore® 4B

• BioWorld\'s Mannose Separopore® 4B was prepared by covalent linkage of D-Mannose to Separopore® 4B via the hydroxyl groups. The likely site for coupling is at the C-6 position.
• Mannose Separopore® may be used to isolate mannose-binding proteins such as the lectin Concanavalin A (Cat # 509940 and 509941).
• Similar resins can be used for lectin purification. Please consult literature for suggested buffers and optimal pH for specific proteins.
• Mannose Separopore® 4B is supplied as an aqueous suspension in 20 % ethanol.

Technical Specificatons

• Ligand: D-Mannose.
• Ligand coupling method: Covalent lnkage.
• Binding capacity: 50-70 mg/ml.
• Matrix:agarose, 4% beads.
• Spacer arm:5 atoms.
• Partcle size (range):50-175µm.
• Storage:2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Mannose Separopore® 4B |Cat# 20181019-2 |Size 25mL EA | $ 184

Mannose Separopore® 4B

• BioWorld\'s Mannose Separopore® 4B was prepared by covalent linkage of D-Mannose to Separopore® 4B via the hydroxyl groups. The likely site for coupling is at the C-6 position.
• Mannose Separopore® may be used to isolate mannose-binding proteins such as the lectin Concanavalin A (Cat # 509940 and 509941).
• Similar resins can be used for lectin purification. Please consult literature for suggested buffers and optimal pH for specific proteins.
• Mannose Separopore® 4B is supplied as an aqueous suspension in 20 % ethanol.

Technical Specificatons

• Ligand: D-Mannose.
• Ligand coupling method: Covalent lnkage.
• Binding capacity: 50-70 mg/ml.
• Matrix:agarose, 4% beads.
• Spacer arm:5 atoms.
• Partcle size (range):50-175µm.
• Storage:2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Mannose Separopore® 4B |Cat# 20181019-3 |Size 50mL EA | $ 297

Mannose Separopore® 4B

• BioWorld\'s Mannose Separopore® 4B was prepared by covalent linkage of D-Mannose to Separopore® 4B via the hydroxyl groups. The likely site for coupling is at the C-6 position.
• Mannose Separopore® may be used to isolate mannose-binding proteins such as the lectin Concanavalin A (Cat # 509940 and 509941).
• Similar resins can be used for lectin purification. Please consult literature for suggested buffers and optimal pH for specific proteins.
• Mannose Separopore® 4B is supplied as an aqueous suspension in 20 % ethanol.

Technical Specificatons

• Ligand: D-Mannose.
• Ligand coupling method: Covalent lnkage.
• Binding capacity: 50-70 mg/ml.
• Matrix:agarose, 4% beads.
• Spacer arm:5 atoms.
• Partcle size (range):50-175µm.
• Storage:2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Mannose Separopore® 4B |Cat# 20181019-4 |Size 100mL EA | $ 398.5

Mannose Separopore® 4B

• BioWorld\'s Mannose Separopore® 4B was prepared by covalent linkage of D-Mannose to Separopore® 4B via the hydroxyl groups. The likely site for coupling is at the C-6 position.
• Mannose Separopore® may be used to isolate mannose-binding proteins such as the lectin Concanavalin A (Cat # 509940 and 509941).
• Similar resins can be used for lectin purification. Please consult literature for suggested buffers and optimal pH for specific proteins.
• Mannose Separopore® 4B is supplied as an aqueous suspension in 20 % ethanol.

Technical Specificatons

• Ligand: D-Mannose.
• Ligand coupling method: Covalent lnkage.
• Binding capacity: 50-70 mg/ml.
• Matrix:agarose, 4% beads.
• Spacer arm:5 atoms.
• Partcle size (range):50-175µm.
• Storage:2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Metal Ion-Chelating Separopore® (metal-free) |Cat# 20181020-1 |Size 1mL 5/PK | $ 88.29

Metal-Chelating Separopore®

• Affinity matrix for proteins with metal bnding properties with exposed amino acids such as histidine and cysteine.
• Can be charged with Zn, Cu, Co and Ni metal ions.
• Imminodiacetate immobilized on the matrix allows versatile chelation of divalent metal ions of choice.
• Ideal for purification of recombinant proteins containing (His)₆ fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked agarose, 6% beads
• Particle size (range): 50-175µm
• Metal ion capacity: > 20µmol Zn²⁺/ml gel
• Binding capacity: > 51mg (His)₆-tagged protein (Mr 29kDa)/ml
• Spacer arm: 14 atom
• pH stability: 3-12
• Storage: 4-30 ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Metal Ion-Chelating Separopore® (metal-free) |Cat# 20181020-2 |Size 10mL EA | $ 89.67

Metal-Chelating Separopore®

• Affinity matrix for proteins with metal bnding properties with exposed amino acids such as histidine and cysteine.
• Can be charged with Zn, Cu, Co and Ni metal ions.
• Imminodiacetate immobilized on the matrix allows versatile chelation of divalent metal ions of choice.
• Ideal for purification of recombinant proteins containing (His)₆ fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked agarose, 6% beads
• Particle size (range): 50-175µm
• Metal ion capacity: > 20µmol Zn²⁺/ml gel
• Binding capacity: > 51mg (His)₆-tagged protein (Mr 29kDa)/ml
• Spacer arm: 14 atom
• pH stability: 3-12
• Storage: 4-30 ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Metal Ion-Chelating Separopore® (metal-free) |Cat# 20181020-3 |Size 25mL EA | $ 164.37

Metal-Chelating Separopore®

• Affinity matrix for proteins with metal bnding properties with exposed amino acids such as histidine and cysteine.
• Can be charged with Zn, Cu, Co and Ni metal ions.
• Imminodiacetate immobilized on the matrix allows versatile chelation of divalent metal ions of choice.
• Ideal for purification of recombinant proteins containing (His)₆ fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked agarose, 6% beads
• Particle size (range): 50-175µm
• Metal ion capacity: > 20µmol Zn²⁺/ml gel
• Binding capacity: > 51mg (His)₆-tagged protein (Mr 29kDa)/ml
• Spacer arm: 14 atom
• pH stability: 3-12
• Storage: 4-30 ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Metal Ion-Chelating Separopore® (metal-free) |Cat# 20181020-4 |Size 3mL 5/PK | $ 168.37

Metal-Chelating Separopore®

• Affinity matrix for proteins with metal bnding properties with exposed amino acids such as histidine and cysteine.
• Can be charged with Zn, Cu, Co and Ni metal ions.
• Imminodiacetate immobilized on the matrix allows versatile chelation of divalent metal ions of choice.
• Ideal for purification of recombinant proteins containing (His)₆ fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked agarose, 6% beads
• Particle size (range): 50-175µm
• Metal ion capacity: > 20µmol Zn²⁺/ml gel
• Binding capacity: > 51mg (His)₆-tagged protein (Mr 29kDa)/ml
• Spacer arm: 14 atom
• pH stability: 3-12
• Storage: 4-30 ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Metal Ion-Chelating Separopore® (metal-free) |Cat# 20181020-5 |Size 50mL EA | $ 301.74

Metal-Chelating Separopore®

• Affinity matrix for proteins with metal bnding properties with exposed amino acids such as histidine and cysteine.
• Can be charged with Zn, Cu, Co and Ni metal ions.
• Imminodiacetate immobilized on the matrix allows versatile chelation of divalent metal ions of choice.
• Ideal for purification of recombinant proteins containing (His)₆ fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked agarose, 6% beads
• Particle size (range): 50-175µm
• Metal ion capacity: > 20µmol Zn²⁺/ml gel
• Binding capacity: > 51mg (His)₆-tagged protein (Mr 29kDa)/ml
• Spacer arm: 14 atom
• pH stability: 3-12
• Storage: 4-30 ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

N-Acetyl-galactosamine Separopore® |Cat# 20181021-1 |Size 2mL EA | $ 214.38

N-acetyl-galactosamine Separopore® n-acetyl-galactosamine is conjugated to Separopore®.

N-Acetyl-galactosamine Separopore® |Cat# 20181021-2 |Size 5mL EA | $ 414.54

N-acetyl-d-galactosamine Separopore® N-acetyl-d-galactosamine is linked to separopore®.

N-Acetyl-galactosamine Separopore® |Cat# 20181021-3 |Size 10mL EA | $ 725.54

N-acetyl-d-galactosamine Separopore® N-acetyl-d-galactosamine is linked to separopore®.

N-Acetyl-glucosamine Separopore® |Cat# 20181022-1 |Size 2mL EA | $ 214.34

N-acetyl-glucosamine Separopore® n-acetyl-glucosamine is conjugated to Separopore®.

N-Acetyl-glucosamine Separopore® |Cat# 20181022-2 |Size 5mL EA | $ 414.18

N-acetyl-glucosamine Separopore® n-acetyl-glucosamine is conjugated to Separopore®.

N-Acetyl-glucosamine Separopore® |Cat# 20181022-3 |Size 10mL EA | $ 725.03

N-acetyl-glucosamine Separopore® n-acetyl-glucosamine is conjugated to Separopore®.

NADP-Separopore® 4B, Lyophilized |Cat# 20181023-1 |Size 1g EA | $ 279.63

NADP Separopore®

NADP covalently bound to Separopore® has a high affinity for NADP- dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP Separopore® twelve times higher than NAD-Separopore®. This has also been used to bind extracellular nucleases.

Specifications:

• Ligand coupling method: CNBr activation
• Matrix attachment: N-6
• Ligand density: 2µmol NADP/ml drained gel
• Particle size: 50-175µm
• pH stability: 4-10
• Storage: -20 °C

• Supplied as lyophilized powder stabilized with lactose,

• Swelling: 1 g swells to approx. 5 ml.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

NADP-Separopore® 4B, Lyophilized |Cat# 20181023-2 |Size 2g EA | $ 413.4

NADP Separopore®

NADP covalently bound to Separopore® has a high affinity for NADP- dependent enzymes and dehydrogenases. Acetyl-CoA binds to NADP Separopore® twelve times higher than NAD-Separopore®. This has also been used to bind extracellular nucleases.

Specifications:

• Ligand coupling method: CNBr activation
• Matrix attachment: N-6
• Ligand density: 2µmol NADP/ml drained gel
• Particle size: 50-175µm
• pH stability: 4-10
• Storage: -20 °C

• Supplied as lyophilized powder stabilized with lactose,

• Swelling: 1 g swells to approx. 5 ml.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

NAD-Separopore® 4B, Lyophilized |Cat# 20181024-1 |Size 1g EA | $ 267.25

NAD-Separopore^®

NAD covalently bound to Separopore®has a high affinity for NAD- dependent enzymes and dehydrogenases. NAD Separopore® has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

Specifications:

Ligand coupling method: CNBr activation

Matrix attachment: N-6 

Ligand density: 2µmol NAD/ml drained gel.

Particle size: 50-160µm

pH stability: 4-10

Storage: -20°C 

Supplied as lyophilized powder stabilized with lactose

Swelling: 1 g swells to approx. 5 ml.

 Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

Reference:

Antoni G, Brizzi A, Casagli MC and Neri P. Ital J Biochem. 1978 Nov-Dec;27(6):373-7.

Cukor G, Nowak NA. J Bacteriol. 1982 Mar;149(3):1162-5.

NAD-Separopore® 4B, Lyophilized |Cat# 20181024-2 |Size 2g EA | $ 413.25

NAD-Separopore^®

NAD covalently bound to Separopore®has a high affinity for NAD- dependent enzymes and dehydrogenases. NAD Separopore® has been used to purify Vibrio cholerae toxin, diptheria toxin and other NAD binding proteins.

Specifications:

Ligand coupling method: CNBr activation

Matrix attachment: N-6 

Ligand density: 2µmol NAD/ml drained gel.

Particle size: 50-160µm

pH stability: 4-10

Storage: -20°C 

Supplied as lyophilized powder stabilized with lactose

Swelling: 1 g swells to approx. 5 ml.

 Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

Reference:

Antoni G, Brizzi A, Casagli MC and Neri P. Ital J Biochem. 1978 Nov-Dec;27(6):373-7.

Cukor G, Nowak NA. J Bacteriol. 1982 Mar;149(3):1162-5.

NHS-activated Separopore® 4B |Cat# 20181025-1 |Size 5mL EA | $ 56.01

NHS-activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide esters to form a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Supplied as a pre-swelled gel suspension in 100% Isopropanol to protect the active groups.

Technical Specifications

• Group to be coupled: Primary -NH2.
• Chemical linkage of ligand: amide bond.
• Matrix: highly cross-linked agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-aminocaproic acid, 14 atom. 
• Substitution:»18 µmol NHS/ml medium.
• pH stability: 3-13 (ligand dependent).
• Coupling conditions: pH 6-9; 4-25ºC.
• Storage: 2-8ºC.
• Typical flow rate » 150 cm/h.
• Storage 100% isopropanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

NHS-activated Separopore® 4B |Cat# 20181025-2 |Size 10mL EA | $ 96.73

NHS-activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide esters to form a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Supplied as a pre-swelled gel suspension in 100% Isopropanol to protect the active groups.

Technical Specifications

• Group to be coupled: Primary -NH2.
• Chemical linkage of ligand: amide bond.
• Matrix: highly cross-linked agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-aminocaproic acid, 14 atom. 
• Substitution:»18 µmol NHS/ml medium.
• pH stability: 3-13 (ligand dependent).
• Coupling conditions: pH 6-9; 4-25ºC.
• Storage: 2-8ºC.
• Typical flow rate » 150 cm/h.
• Storage 100% isopropanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

NHS-activated Separopore® 4B |Cat# 20181025-3 |Size 25mL EA | $ 153.4

NHS-activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide esters to form a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Supplied as a pre-swelled gel suspension in 100% Isopropanol to protect the active groups.

Technical Specifications

• Group to be coupled: Primary -NH2.
• Chemical linkage of ligand: amide bond.
• Matrix: highly cross-linked agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-aminocaproic acid, 14 atom. 
• Substitution:»18 µmol NHS/ml medium.
• pH stability: 3-13 (ligand dependent).
• Coupling conditions: pH 6-9; 4-25ºC.
• Storage: 2-8ºC.
• Typical flow rate » 150 cm/h.
• Storage 100% isopropanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

NHS-activated Separopore® 4B |Cat# 20181025-4 |Size 50mL EA | $ 222.71

NHS-activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide esters to form a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Supplied as a pre-swelled gel suspension in 100% Isopropanol to protect the active groups.

Technical Specifications

• Group to be coupled: Primary -NH2.
• Chemical linkage of ligand: amide bond.
• Matrix: highly cross-linked agarose, 4% beads.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 90 µm.
• Spacer arm: 6-aminocaproic acid, 14 atom. 
• Substitution:»18 µmol NHS/ml medium.
• pH stability: 3-13 (ligand dependent).
• Coupling conditions: pH 6-9; 4-25ºC.
• Storage: 2-8ºC.
• Typical flow rate » 150 cm/h.
• Storage 100% isopropanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Papain Separopore® 4B |Cat# 20181027-1 |Size 5mL EA | $ 187.84

Specifications:

• Matrix: Cross-linked 4% Agarose beads , Separopore® 4B
• Activity: 16-40 BAEE units per milligram of papain before immobilization
• Loading: 250mg papain/ml of settled gel
• Particle size (range): 63-150 µm
• Supplied as: 50% slurry in 50% glycerol, 0.1M Sodium acetate ( pH 4.4) containing    0.05% Sodium azide

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

Papain Separopore® 4B |Cat# 20181027-2 |Size 10mL EA | $ 310.44

Specifications:

• Matrix: Cross-linked 4% Agarose beads , Separopore® 4B
• Activity: 16-40 BAEE units per milligram of papain before immobilization
• Loading: 250mg papain/ml of settled gel
• Particle size (range): 63-150 µm
• Supplied as: 50% slurry in 50% glycerol, 0.1M Sodium acetate ( pH 4.4) containing    0.05% Sodium azide

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

Protein A Separopore® (Lyophilized) |Cat# 20181028-1 |Size 1g EA | $ 263.09

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• supplied as lyophilized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus
• Ligand coupling method: CNBr activation
• Binding capacity: approximately 20mg human IgG/ml drained gel
• Matrix: agarose, 4%
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-165µ
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8ºC

Protein A Separopore® (Lyophilized) |Cat# 20181028-2 |Size 5g EA | $ 938.2

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• supplied as lyophilized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus
• Ligand coupling method: CNBr activation
• Binding capacity: approximately 20mg human IgG/ml drained gel
• Matrix: agarose, 4%
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-165µ
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8ºC

Protein A Separopore® 4B |Cat# 20181029-1 |Size 0.8mL 2/PK | $ 98.7

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl or as lyophylized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 45-165µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein A Separopore® 4B |Cat# 20181029-2 |Size 2mL EA | $ 159.7

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl or as lyophylized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 45-165µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein A Separopore® 4B |Cat# 20181029-3 |Size 5mL EA | $ 285.6

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl or as lyophylized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 45-165µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein A Separopore® 4B |Cat# 20181029-4 |Size 10mL EA | $ 495

Protein A Separopore® 4B

• Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl or as lyophylized powder.

Technical Specifications

• Ligand: Native protein A from Staphylococcus aureus.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 45-165µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein-G Separopore® 4B |Cat# 20181030-1 |Size 1mL EA | $ 142.85

Protein-G Separopore® 4B

• Affinity matrix with immobilized protein-G (from Streptococcus sp.) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.
• Reliable tool to purify monoclonal and polyclonal IgG form ascites, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: native protein-G from Streptococcus sp.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein-G Separopore® 4B |Cat# 20181030-2 |Size 5mL EA | $ 438.69

Protein-G Separopore® 4B

• Affinity matrix with immobilized protein-G (from Streptococcus sp.) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.
• Reliable tool to purify monoclonal and polyclonal IgG form ascites, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: native protein-G from Streptococcus sp.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein-G Separopore® 4B |Cat# 20181030-3 |Size 10mL EA | $ 645.71

Protein-G Separopore® 4B

• Affinity matrix with immobilized protein-G (from Streptococcus sp.) by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.
• Reliable tool to purify monoclonal and polyclonal IgG form ascites, serum and cell culture supernatants.
• Supplied as suspension in 0.5M NaCl.

Technical Specifications

• Ligand: native protein-G from Streptococcus sp.
• Ligand coupling method: CNBr activation.
• Binding capacity: approximately 20mg human IgG/ml drained gel.
• Matrix: agarose, 4%.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-9 (for use), 2-10 (for cleaning).
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 4B |Cat# 20181031-1 |Size 100mL EA | $ 98.7

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose 4% Beads
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B |Cat# 20181031-2 |Size 500mL EA | $ 306.41

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose 4% Beads
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B |Cat# 20181031-3 |Size 1L EA | $ 568.63

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose 4% Beads
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-CL |Cat# 20181032-1 |Size 50mL EA | $ 79.03

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 45-165 µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose, 4% cross-linked Beads
• pH stability: 3-12
• Flow specifications: 80-150 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-CL |Cat# 20181032-2 |Size 100mL EA | $ 111.6

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 45-165 µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose, 4% cross-linked Beads
• pH stability: 3-12
• Flow specifications: 80-150 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-CL |Cat# 20181032-3 |Size 500mL EA | $ 321.7

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 45-165 µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose, 4% cross-linked Beads
• pH stability: 3-12
• Flow specifications: 80-150 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-CL |Cat# 20181032-4 |Size 1L EA | $ 598.7

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 45-165 µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Agarose, 4% cross-linked Beads
• pH stability: 3-12
• Flow specifications: 80-150 cm/h
• Supplied as a slurry in 20% ethanol

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-MB (Macrobeads) |Cat# 20181033-1 |Size 100mL EA | $ 131.04

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 4% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-MB (Macrobeads) |Cat# 20181033-2 |Size 500mL EA | $ 372.12

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 4% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 4B-MB (Macrobeads) |Cat# 20181033-3 |Size 1L EA | $ 641.28

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 4% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-MB |Cat# 20181035-1 |Size 100mL EA | $ 149.11

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 6% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-MB |Cat# 20181035-2 |Size 500mL EA | $ 392.56

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 6% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-MB |Cat# 20181035-3 |Size 1L EA | $ 660.28

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm
• Matrix: Agarose 6% beads
• pH stability: 4-9

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-CL |Cat# 20181036-1 |Size 50mL EA | $ 86.93

A cross-linked high strength agarose medium for gel filtration and coupling of affinity ligands. A hydrophilic backbone results in low non specific interactions and high yields. Excellent separation over a wide molecular weight range.

Spefications:

• Particle Size (range): 50-175 µm
• Molecular Weight range: 6x104 - 2x107
• atrix: Cross-linked agarose 6% beads
• pH stability: 3-12
• low specifications: 100-180cm/h

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-CL |Cat# 20181036-2 |Size 100mL EA | $ 128.45

A cross-linked high strength agarose medium for gel filtration and coupling of affinity ligands. A hydrophilic backbone results in low non specific interactions and high yields. Excellent separation over a wide molecular weight range.

Spefications:

• Particle Size (range): 50-175 µm
• Molecular Weight range: 6x104 - 2x107
• atrix: Cross-linked agarose 6% beads
• pH stability: 3-12
• low specifications: 100-180cm/h

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-CL |Cat# 20181036-3 |Size 500mL EA | $ 374.71

A cross-linked high strength agarose medium for gel filtration and coupling of affinity ligands. A hydrophilic backbone results in low non specific interactions and high yields. Excellent separation over a wide molecular weight range.

Spefications:

• Particle Size (range): 50-175 µm
• Molecular Weight range: 6x104 - 2x107
• atrix: Cross-linked agarose 6% beads
• pH stability: 3-12
• low specifications: 100-180cm/h

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 6B-CL |Cat# 20181036-4 |Size 1L EA | $ 679.7

A cross-linked high strength agarose medium for gel filtration and coupling of affinity ligands. A hydrophilic backbone results in low non specific interactions and high yields. Excellent separation over a wide molecular weight range.

Spefications:

• Particle Size (range): 50-175 µm
• Molecular Weight range: 6x104 - 2x107
• atrix: Cross-linked agarose 6% beads
• pH stability: 3-12
• low specifications: 100-180cm/h

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Streptavidin Separopore® |Cat# 20181037-1 |Size 1mL EA | $ 60.73

Streptavidin Separopore®

• Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
• For purification of biotinylated compounds.
• Supplied as buffered saline solution.

Technical specifications

• Ligand: Native Streptavidin (from Streptomyces vidinii).
• Ligand coupling method: CNBr activation.
• Ligand density: 2mg/ml.
• Binding capacity: >20µg biotin/ml drained gel.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-10.
• Binding buffer: 50mM ammonium carbonate, 0.5M NaCl, pH 10.0.
• Elution buffer: 50mM ammonium acetate, 0.5M NaCl, pH 4.0.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Streptavidin Separopore® |Cat# 20181037-2 |Size 5mL EA | $ 214.9

Streptavidin Separopore®

• Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
• For purification of biotinylated compounds.
• Supplied as buffered saline solution.

Technical specifications

• Ligand: Native Streptavidin (from Streptomyces vidinii).
• Ligand coupling method: CNBr activation.
• Ligand density: 2mg/ml.
• Binding capacity: >20µg biotin/ml drained gel.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-10.
• Binding buffer: 50mM ammonium carbonate, 0.5M NaCl, pH 10.0.
• Elution buffer: 50mM ammonium acetate, 0.5M NaCl, pH 4.0.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Streptavidin Separopore® |Cat# 20181037-3 |Size 10mL EA | $ 468.38

Streptavidin Separopore®

• Affinity matrix with immobilized Streptavidin by CNBr activation coupling method.
• For purification of biotinylated compounds.
• Supplied as buffered saline solution.

Technical specifications

• Ligand: Native Streptavidin (from Streptomyces vidinii).
• Ligand coupling method: CNBr activation.
• Ligand density: 2mg/ml.
• Binding capacity: >20µg biotin/ml drained gel.
• Matrix: agarose, 4% beads.
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm.
• pH stability: 3-10.
• Binding buffer: 50mM ammonium carbonate, 0.5M NaCl, pH 10.0.
• Elution buffer: 50mM ammonium acetate, 0.5M NaCl, pH 4.0.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Thiophilic Separopore® |Cat# 20181038-1 |Size 5mL EA | $ 86.5

The term \"Thiophilic\" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that

Thiophilic Separopore® |Cat# 20181038-2 |Size 10mL EA | $ 138.7

The term \"Thiophilic\" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that

Thiophilic Separopore® |Cat# 20181038-3 |Size 25mL EA | $ 298

The term \"Thiophilic\" refers to an affinity for sulfone groups that lie in close proximity to thioether groups. Thiophilic affinity purification is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that

Tosyl-activated Separopore® |Cat# 20181039-1 |Size 10mL EA | $ 49.26

Tosyl-activated Separopore®

Can be used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups. Tosyl-activated Separopore® couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interferring with the antigen binding region.

Specifications:

• Matrix Separopore® 4B (4 % Agarose Beads).
• Partcle Size 50-175µm.
• Couples to primary amines at pH range 9-10.
• Phosphate, HEPES, bicarbonate and borate buffers should be used.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Tosyl-activated Separopore® |Cat# 20181039-2 |Size 50mL EA | $ 132.75

Tosyl-activated Separopore®

Can be used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups. Tosyl-activated Separopore® couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interferring with the antigen binding region.

Specifications:

• Matrix Separopore® 4B (4 % Agarose Beads).
• Partcle Size 50-175µm.
• Couples to primary amines at pH range 9-10.
• Phosphate, HEPES, bicarbonate and borate buffers should be used.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Tosyl-activated Separopore® |Cat# 20181039-3 |Size 100mL EA | $ 216.5

Tosyl-activated Separopore®

Can be used to couple primary amines, thiols, imidazole or tyrosine hydroxyl groups. Tosyl-activated Separopore® couples more rapidly and efficiently to sulfhydryl groups than to primary amines. Coupling of antibodies through reduced disulfide bounds in the hinge region is a popular way to couple without interferring with the antigen binding region.

Specifications:

• Matrix Separopore® 4B (4 % Agarose Beads).
• Partcle Size 50-175µm.
• Couples to primary amines at pH range 9-10.
• Phosphate, HEPES, bicarbonate and borate buffers should be used.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ubiquitin Separopore® |Cat# 20181040-1 |Size 2x1mL 2/PK | $ 121.9

Ubiquitin-Separopore®
Covalently linked ubiquitin to Separopore® beads via primary amine coupling leaving the C-terminus free. Useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases.

Specifications:

Matrix: Separopore® 4B (4% Agarose beads).

Particle size: 50-175µm.

Binding Capacity: 2mg/ml resin.

Storage: 4°C.

Supplied in a 20% ethanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ubiquitin Separopore® |Cat# 20181040-2 |Size 10mL EA | $ 219.34

Ubiquitin-Separopore®
Covalently linked ubiquitin to Separopore® beads via primary amine coupling leaving the C-terminus free. Useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases.

Specifications:

Matrix: Separopore® 4B (4% Agarose beads).

Particle size: 50-175µm.

Binding Capacity: 2mg/ml resin.

Storage: 4°C.

Supplied in a 20% ethanol.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

View-IP Protein A Red Separopore® |Cat# 20181041-1 |Size 2mL EA | $ 179.67

View-IP Protein-A Red Separopore® CL-4B

For quantitative capturing of antigen/antibody complexes in immunoprecipitation

• This colored Affinity matrix has immobilized Recombinant Protein-A
• Coupled by CNBr activation method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as 50% suspension in PBS, containing 50% glycerol.

Technical Specifications

Ligand: Recombinant Protein A from Staphylococcus aureus

Ligand coupling method: CNBr activation

Binding capacity: approximately 20mg human IgG/ml drained gel

Matrix: crosslinked agarose, 4%

Exclusion limit: approximately 2 x 10⁷

Particle size (range): 50-165µm

pH stability: 3-9 (for use), 2-10 (for cleaning)

Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

View-IP Protein A Red Separopore® |Cat# 20181041-2 |Size 5mL EA | $ 394.5

View-IP Protein-A Red Separopore® CL-4B

For quantitative capturing of antigen/antibody complexes in immunoprecipitation

• This colored Affinity matrix has immobilized Recombinant Protein-A
• Coupled by CNBr activation method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• Supplied as 50% suspension in PBS, containing 50% glycerol.

Technical Specifications

Ligand: Recombinant Protein A from Staphylococcus aureus

Ligand coupling method: CNBr activation

Binding capacity: approximately 20mg human IgG/ml drained gel

Matrix: crosslinked agarose, 4%

Exclusion limit: approximately 2 x 10⁷

Particle size (range): 50-165µm

pH stability: 3-9 (for use), 2-10 (for cleaning)

Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

 

Protein A Blue Separopore® 4B (Macrobeads) |Cat# 20181042-1 |Size 2mL EA | $ 179.67

Protein A Blue Separopore® 4B (Macrobeads)

• Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• supplied as suspension in 0.5M NaCl, 0.02% sodium azide.

Technical Specifications

• Ligand: Recombinant protein A
• Ligand coupling method: CNBr activation
• Binding capacity: approximately 20mg human IgG/ml drained gel
• Matrix: agarose, 4%
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 150-275µm
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein A Blue Separopore® 4B (Macrobeads) |Cat# 20181042-2 |Size 5mL EA | $ 395.16

Protein A Blue Separopore® 4B (Macrobeads)

• Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• supplied as suspension in 0.5M NaCl, 0.02% sodium azide.

Technical Specifications

• Ligand: Recombinant protein A
• Ligand coupling method: CNBr activation
• Binding capacity: approximately 20mg human IgG/ml drained gel
• Matrix: agarose, 4%
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 150-275µm
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Protein A Blue Separopore® 4B (Macrobeads) |Cat# 20181042-3 |Size 10mL EA | $ 464.5

Protein A Blue Separopore® 4B (Macrobeads)

• Affinity matrix with immobilized Protein-A by CNBr activation coupling method.
• For purification and/or fractionation of IgG subclasses.
• Reliable tool to purify monoclonal and polyclonal IgG from ascies, serum and cell culture supernatants.
• supplied as suspension in 0.5M NaCl, 0.02% sodium azide.

Technical Specifications

• Ligand: Recombinant protein A
• Ligand coupling method: CNBr activation
• Binding capacity: approximately 20mg human IgG/ml drained gel
• Matrix: agarose, 4%
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 150-275µm
• pH stability: 3-9 (for use), 2-10 (for cleaning)
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 6B |Cat# 20181043-1 |Size 100mL EA | $ 108.45

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 4x10⁶

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol
Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 6B |Cat# 20181043-2 |Size 500mL EA | $ 368.55

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 4x10⁶

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol
Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 6B |Cat# 20181043-3 |Size 1L EA | $ 657.31

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 4x10⁶

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol
Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® (Microbeads) |Cat# 20181044-1 |Size 10mL EA | $ 119.43

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 107
• Particle size (range): 20-50µm

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® (Microbeads) |Cat# 20181044-2 |Size 25mL EA | $ 237.33

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 107
• Particle size (range): 20-50µm

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 2B |Cat# 20181045-1 |Size 100mL EA | $ 95.89

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications

 

• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Agarose 2% Beads.
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a slurry in 20% ethanol.
• Typical flow rate 10cm/h.
• Chemical stability Stable to all solutions commonly used in gel filtration including 8 M Urea 6 M guanidine hydrochloride.
• Physical stability Negligible volume variation due to changes in pH or ionic strength.
• Sterilization Chemical.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B |Cat# 20181045-2 |Size 500mL EA | $ 301.21

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications

 

• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Agarose 2% Beads.
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a slurry in 20% ethanol.
• Typical flow rate 10cm/h.
• Chemical stability Stable to all solutions commonly used in gel filtration including 8 M Urea 6 M guanidine hydrochloride.
• Physical stability Negligible volume variation due to changes in pH or ionic strength.
• Sterilization Chemical.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B |Cat# 20181045-3 |Size 1L EA | $ 561.36

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications

 

• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Agarose 2% Beads.
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a slurry in 20% ethanol.
• Typical flow rate 10cm/h.
• Chemical stability Stable to all solutions commonly used in gel filtration including 8 M Urea 6 M guanidine hydrochloride.
• Physical stability Negligible volume variation due to changes in pH or ionic strength.
• Sterilization Chemical.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Ni-Chelated Separopore® |Cat# 20181046-1 |Size 10mL EA | $ 95

Ni-Chelated Separopore® is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.

Properties:

• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore®6% beads
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Dynamic bInding Capacity: Approx. 40 mg histidine-tagged protein/ml medium 
• Chemical Stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS 
• Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ni-Chelated Separopore® |Cat# 20181046-2 |Size 25mL EA | $ 195

Ni-Chelated Separopore® is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.

Properties:

• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore®6% beads
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Dynamic bInding Capacity: Approx. 40 mg histidine-tagged protein/ml medium 
• Chemical Stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS 
• Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ni-Chelated Separopore® |Cat# 20181046-3 |Size 50mL EA | $ 337

Ni-Chelated Separopore® is an imminodiacetate (IDA)-immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.

Properties:

• Imminodiacetate immobilized matrix charged with Ni metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid
• Chelating group coupling method: epoxy activation
• Matrix: cross-linked Separopore®6% beads
• Particle size (range): 50-170µm
• Metal ion capacity: > 20µmol Ni2+/ml gel
• Spacer arm: 14 atom
• pH stability: 3-12
• Dynamic bInding Capacity: Approx. 40 mg histidine-tagged protein/ml medium 
• Chemical Stability For one week at 40°C: 0.01 M HCl, 0.1 M NaOH For 12 h: 1 M NaOH, 70% acetic acid 30 min tested: 30% 2-propanol 1 h tested: 2% SDS 
• Compatibility during use Stable in all commonly used buffers, reducing agents, denaturants, and detergents

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Co-Chelated Separopore® |Cat# 20181047-1 |Size 10mL EA | $ 95

Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.
 
Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Co metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Co2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.
• Chem. Stability 40°C for 7 days in: 0.01 M HCl, 1 M NaOH, acetate buffer pH 4,20% ethanol. 
• Autoclavable At 121°C, 20% ethanol. 
• Antimicrobial agent 20% ethanol. 
• Chelating Gp. Coup. Linkage Ether. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Co-Chelated Separopore® |Cat# 20181047-2 |Size 25mL EA | $ 195

Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.
 
Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Co metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Co2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.
• Chem. Stability 40°C for 7 days in: 0.01 M HCl, 1 M NaOH, acetate buffer pH 4,20% ethanol. 
• Autoclavable At 121°C, 20% ethanol. 
• Antimicrobial agent 20% ethanol. 
• Chelating Gp. Coup. Linkage Ether. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Co-Chelated Separopore® |Cat# 20181047-3 |Size 50mL EA | $ 337

Co-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to nickel metal ions.
 
Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Co metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Co2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.
• Chem. Stability 40°C for 7 days in: 0.01 M HCl, 1 M NaOH, acetate buffer pH 4,20% ethanol. 
• Autoclavable At 121°C, 20% ethanol. 
• Antimicrobial agent 20% ethanol. 
• Chelating Gp. Coup. Linkage Ether. 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Cu-Chelated Separopore® |Cat# 20181048-1 |Size 10mL EA | $ 95

Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Cu metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Cu2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Cu-Chelated Separopore® |Cat# 20181048-2 |Size 25mL EA | $ 195

Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Cu metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Cu2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Cu-Chelated Separopore® |Cat# 20181048-3 |Size 50mL EA | $ 337

Cu-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Cu metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Cu2+/ml gel.
• Spacer arm: 14 atom.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Zn-Chelated Separopore® |Cat# 20181049-1 |Size 10mL EA | $ 95

Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Zn metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Zn2+/ml gel.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Zn-Chelated Separopore® |Cat# 20181049-2 |Size 25mL EA | $ 195

Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Zn metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Zn2+/ml gel.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Zn-Chelated Separopore® |Cat# 20181049-3 |Size 50mL EA | $ 337

Zn-Chelated Separopore® is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Zn metal ions.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Zn metal ions.
• Ideal for purification of recombinant proteins containing (His)6 fusion tag.
• Supplied as suspension in 20% ethanol.

Technical Specifications

• Chelating group: Imminodiacetic acid.
• Chelating group coupling method: epoxy activation.
• Matrix: cross-linked Separopore®, 4% beads.
• Particle size (range): 50-175µm.
• Metal ion capacity: > 20µmol Zn2+/ml gel.
• pH stability: 3-12.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® |Cat# 20181050-1 |Size 10mL EA | $ 108.57

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Glutathione Separopore® |Cat# 20181050-2 |Size 25mL EA | $ 215.754

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Glutathione Separopore® |Cat# 20181050-3 |Size 50mL EA | $ 497

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Glutathione Separopore |Cat# 20181050-4 |Size 100mL EA | $ 799.304

Specifications

• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

ATP Separopore® |Cat# 20181051-1 |Size 5mL EA | $ 198.5

Adenosine 5\'-triphosphate (ATP) Separapore™ for use in purification of ATP binding proteins.

Technical Specification:

• Matrix: Cross-linked Agarose beads, 4%.
• Particle size range: 50-175μm.
• Activation: Cyanogen bromide.
• Attachement: N-6 of the purine ring.
• Spacer: 11 atoms.
• Ligand: ATP.
• Ligand density: 2-5 μmol ATP/ml drained matrix.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ATP Separopore® |Cat# 20181051-2 |Size 10mL EA | $ 357

Adenosine 5\'-triphosphate (ATP) Separapore™ for use in purification of ATP binding proteins.

Technical Specification:

• Matrix: Cross-linked Agarose beads, 4%.
• Particle size range: 50-175μm.
• Activation: Cyanogen bromide.
• Attachement: N-6 of the purine ring.
• Spacer: 11 atoms.
• Ligand: ATP.
• Ligand density: 2-5 μmol ATP/ml drained matrix.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Guar gum Separopore® 4B-CL |Cat# 20181052-1 |Size 10mL EA | $ 89.5

Guar gum is a Galactomannan polymer derived from the seeds of the legume plant Cyamopsis tetragonolobus.Guar gum based affinity chromatography is used for the purification of galactose binding proteins such as lectins.

Technical Specificatons

• Matrix: Cross-linked Agarose, 4% beads, Separopore® 4B-CL.
• Particle size (range): 50-150µm.
• Ligand: Guargum.
• Ligand coupling method: Epoxy activation.
• Binding capacity: 4 mg/ml.
• Supplied as an aqueous suspension in 20 % ethanol.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Guar gum Separopore® 4B-CL |Cat# 20181052-2 |Size 25mL EA | $ 184

Guar gum is a Galactomannan polymer derived from the seeds of the legume plant Cyamopsis tetragonolobus.Guar gum based affinity chromatography is used for the purification of galactose binding proteins such as lectins.

Technical Specificatons

• Matrix: Cross-linked Agarose, 4% beads, Separopore® 4B-CL.
• Particle size (range): 50-150µm.
• Ligand: Guargum.
• Ligand coupling method: Epoxy activation.
• Binding capacity: 4 mg/ml.
• Supplied as an aqueous suspension in 20 % ethanol.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Guar gum Separopore® 4B-CL |Cat# 20181052-3 |Size 50mL EA | $ 297

Guar gum is a Galactomannan polymer derived from the seeds of the legume plant Cyamopsis tetragonolobus.Guar gum based affinity chromatography is used for the purification of galactose binding proteins such as lectins.

Technical Specificatons

• Matrix: Cross-linked Agarose, 4% beads, Separopore® 4B-CL.
• Particle size (range): 50-150µm.
• Ligand: Guargum.
• Ligand coupling method: Epoxy activation.
• Binding capacity: 4 mg/ml.
• Supplied as an aqueous suspension in 20 % ethanol.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Guar gum Separopore® 4B-CL |Cat# 20181052-4 |Size 100mL EA | $ 398

Guar gum is a Galactomannan polymer derived from the seeds of the legume plant Cyamopsis tetragonolobus.Guar gum based affinity chromatography is used for the purification of galactose binding proteins such as lectins.

Technical Specificatons

• Matrix: Cross-linked Agarose, 4% beads, Separopore® 4B-CL.
• Particle size (range): 50-150µm.
• Ligand: Guargum.
• Ligand coupling method: Epoxy activation.
• Binding capacity: 4 mg/ml.
• Supplied as an aqueous suspension in 20 % ethanol.
• Storage: 2-8ºC.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Separopore® 2B-CL |Cat# 20181053-1 |Size 100mL EA | $ 107.7

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 50-175 µm.
• Molecular Weight range: 7×104 - 4×107.
• Matrix: Agarose, 2% cross-linked Beads.
• pH stability: 5-9.
• Flow specifications: 60-120 cm/h.
• Supplied as a slurry in 20% ethanol.
• CIP stability 3 to 13.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B-CL |Cat# 20181053-2 |Size 500mL EA | $ 314.51

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 50-175 µm.
• Molecular Weight range: 7×104 - 4×107.
• Matrix: Agarose, 2% cross-linked Beads.
• pH stability: 5-9.
• Flow specifications: 60-120 cm/h.
• Supplied as a slurry in 20% ethanol.
• CIP stability 3 to 13.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B-CL |Cat# 20181053-3 |Size 1L EA | $ 590.89

A cross-linked bioagarose medium for gel filtration and coupling of affinity ligands. Hydrophilic backbone results in low non-specific interactions and high protein recoveries . Provides excellent separation of proteins over a wide molecular weight range.

Specifications:

• Particle size (range): 50-175 µm.
• Molecular Weight range: 7×104 - 4×107.
• Matrix: Agarose, 2% cross-linked Beads.
• pH stability: 5-9.
• Flow specifications: 60-120 cm/h.
• Supplied as a slurry in 20% ethanol.
• CIP stability 3 to 13.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B-MB (Macrobeads) |Cat# 20181054-1 |Size 100mL EA | $ 115.37

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm.
• Matrix: Agarose 2% beads.
• pH stability: 4-9.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B-MB (Macrobeads) |Cat# 20181054-2 |Size 500mL EA | $ 361.45

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm.
• Matrix: Agarose 2% beads.
• pH stability: 4-9.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Separopore® 2B-MB (Macrobeads) |Cat# 20181054-3 |Size 1L EA | $ 637.14

An agarose bead matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications.

Specifications

• Particle size (range): 150-265µm.
• Matrix: Agarose 2% beads.
• pH stability: 4-9.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

SP Separopore® |Cat# 20181055-1 |Size 100mL EA | $ Call for price

A high gel strength bioagarose matrix for gel filtration and cation exchanger suitable for analytical and preparative protein purifications. The cation exchange group for this product consists of a sulphonyl group effective between a working pH range of 4-13.

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SP Separopore® |Cat# 20181055-2 |Size 500mL EA | $ Call for price

A high gel strength bioagarose matrix for gel filtration and cation exchanger suitable for analytical and preparative protein purifications. The cation exchange group for this product consists of a sulphonyl group effective between a working pH range of 4-13.

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

2-Iminobiotin Separopore® |Cat# 20181056-1 |Size 5mL EA | $ 127.35

Application: For the purification of avidin containing proteins.

Specifications:

Ligand: 2-Iminobiotin

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5M NaCl, 0.01 M sodium phosphate (pH 6.8), 0.02% sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

2-Iminobiotin Separopore® |Cat# 20181056-2 |Size 10mL EA | $ 237.55

Application: For the purification of avidin containing proteins.

Specifications:

Ligand: 2-Iminobiotin

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5M NaCl, 0.01 M sodium phosphate (pH 6.8), 0.02% sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ADP Separopore® |Cat# 20181057-1 |Size 1mL EA | $ 40.77

Specifications:

• Ligand: Adenosine 5\'-diphosphate
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5M NaCl, 0.02% thimerosal.
• Application: For the purification of ADP dependent enzymes and proteins.
• Extent Of Labelling 1-3 μmol per mL
• Matrix Activation cyanogen bromide
• Matrix Attachment ribose hydroxyls
• Matrix Spacer 11 atoms (adipic acid dihydrazide)

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ADP Separopore® |Cat# 20181057-2 |Size 5mL EA | $ 148.05

Specifications:

• Ligand: Adenosine 5\'-diphosphate
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5M NaCl, 0.02% thimerosal.
• Application: For the purification of ADP dependent enzymes and proteins.
• Extent Of Labelling 1-3 μmol per mL
• Matrix Activation cyanogen bromide
• Matrix Attachment ribose hydroxyls
• Matrix Spacer 11 atoms (adipic acid dihydrazide)

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

ADP Separopore® |Cat# 20181057-3 |Size 10mL EA | $ 266.49

Specifications:

• Ligand: Adenosine 5\'-diphosphate
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5M NaCl, 0.02% thimerosal.
• Application: For the purification of ADP dependent enzymes and proteins.
• Extent Of Labelling 1-3 μmol per mL
• Matrix Activation cyanogen bromide
• Matrix Attachment ribose hydroxyls
• Matrix Spacer 11 atoms (adipic acid dihydrazide)

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Aldehyde Separopore® |Cat# 20181058-1 |Size 10mL EA | $ 175.13

Specifications:

• Ligand: 2-Iminobiotin
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Application: For the coupling of various ligands

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Aldehyde Separopore® |Cat# 20181058-2 |Size 25mL EA | $ 289.87

Specifications:

• Ligand: 2-Iminobiotin
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Application: For the coupling of various ligands

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Aldehyde Separopore® |Cat# 20181058-3 |Size 50mL EA | $ 377.26

Specifications:

• Ligand: 2-Iminobiotin
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Application: For the coupling of various ligands

 

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Anti-Bovine Albumin (BSA) Separopore® |Cat# 20181059-1 |Size 1mL EA | $ 129.46

Application: For the purification of Bovine Albumin (BSA).

Specifications:

Ligand: Anti-BSA Antibody

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.01M sodium phosphate (pH 7.0), and 15-20mM sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Anti-Bovine Albumin (BSA) Separopore® |Cat# 20181059-2 |Size 5mL EA | $ 595

Application: For the purification of Bovine Albumin (BSA).

Specifications:

Ligand: Anti-BSA Antibody

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.01M sodium phosphate (pH 7.0), and 15-20mM sodium azide.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Avidin Separopore® |Cat# 20181060-1 |Size 1mL EA | $ 73.35

Specifications:

• Ligand: Avidin (from egg white)
• Particle size (range): 50-175µm
• Molecular Weight range: 1x104 - 4x106
• Matrix: Separopore® 6B-CL (Crosslinked Agarose 6% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.01M sodium phosphate (pH 7.0), and 15-20mM sodium azide.
• Application: For the purification of biotin containing proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Avidin Separopore® |Cat# 20181060-2 |Size 5mL EA | $ 252.9

Specifications:

• Ligand: Avidin (from egg white)
• Particle size (range): 50-175µm
• Molecular Weight range: 1x104 - 4x106
• Matrix: Separopore® 6B-CL (Crosslinked Agarose 6% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.01M sodium phosphate (pH 7.0), and 15-20mM sodium azide.
• Application: For the purification of biotin containing proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Avidin Separopore® |Cat# 20181060-3 |Size 10mL EA | $ 469.22

Specifications:

• Ligand: Avidin (from egg white)
• Particle size (range): 50-175µm
• Molecular Weight range: 1x104 - 4x106
• Matrix: Separopore® 6B-CL (Crosslinked Agarose 6% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.01M sodium phosphate (pH 7.0), and 15-20mM sodium azide.
• Application: For the purification of biotin containing proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Biotin Separopore® |Cat# 20181061-1 |Size 1mL EA | $ 54.61

Application: For the purification of avidin and streptavidin containing proteins.

Specifications:

Ligand: Biotin

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5M NaCl, 0.01% thimerosal.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Biotin Separopore® |Cat# 20181061-2 |Size 5mL EA | $ 187.65

Application: For the purification of avidin and streptavidin containing proteins.

Specifications:

Ligand: Biotin

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5M NaCl, 0.01% thimerosal.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Biotin Separopore® |Cat# 20181061-3 |Size 10mL EA | $ 346.54

Application: For the purification of avidin and streptavidin containing proteins.

Specifications:

Ligand: Biotin

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5M NaCl, 0.01% thimerosal.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 6B |Cat# 20181062-1 |Size 1g EA | $ 69.23

Specifications:

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 6% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 6B |Cat# 20181062-2 |Size 5g EA | $ 193.5

Specifications:

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 6% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

CNBr-activated Separopore® 6B |Cat# 20181062-3 |Size 10g EA | $ 307.84

Specifications:

• Pre-activated medium for immobilization of ligands containing primary amines.
• Reproducible, simple and reliable method for covalent immobilization of ligands containing primary amines.
• Mild coupling condition makes CNBr-activated matrix as the ideal tool for coupling enzymes and antibodies.
• Supplied as lyophilized powder
• 1g swell to approx. 4ml
• Group to be coupled: -NH2
• Chemical linkage of ligand: isourea
• Matrix: Agarose, 6% beads
• pH Stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10, 4-25ºC, 4-16 h
• Storage: 2-8ºC

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

D-Alanine Separopore® |Cat# 20181063-1 |Size 5mL EA | $ 78.03

Specifications:

• Ligand: D-Alanine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of Liposomal proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

D-Alanine Separopore® |Cat# 20181063-2 |Size 10mL EA | $ 140.45

Specifications:

• Ligand: D-Alanine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of Liposomal proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

D-Alanine Separopore® |Cat# 20181063-3 |Size 25mL EA | $ 252.82

Specifications:

• Ligand: D-Alanine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of Liposomal proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Epoxy-activated Separopore® 6B |Cat# 20181064-1 |Size 1g EA | $ 63.89

Epoxy-activated Separopore® 6B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 6B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH₂, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 6% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Epoxy-activated Separopore® 6B |Cat# 20181064-2 |Size 5g EA | $ 293.85

Epoxy-activated Separopore® 6B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 6B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH₂, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 6% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Epoxy-activated Separopore® 6B |Cat# 20181064-3 |Size 10g EA | $ 528.93

Epoxy-activated Separopore® 6B

• Used in coupling of hydroxy, amino or thiol groups to Separopore® 6B via a 12-carbon hydrophilic spacer arm.
• Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
• Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
• Epoxy-activated Separopore® 4B is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
• Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.
• Supplied as lyophilized powder.
• 1g swells to approx. 2.8ml.

Technical Specifications

• Group to be coupled: -NH₂, -OH, or -SH
• Chemical linkage of ligand: ether
• Matrix: 6% agarose, slightly cross-linked
• pH stability: 2-11 (ligand dependent)
• Coupling conditions: pH 9-10 (amino ligands), pH 11-12 (hydroxyl ligands), pH 7.5-8.5 (thiol ligands); 4-37ºC, 16 h-several days; organic solvents possible
• Storage: 2-8°C

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Glycine Separopore® |Cat# 20181065-1 |Size 5mL EA | $ 97.45

Specifications:

• Ligand: Glycine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B (Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of synaptic membrane proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glycine Separopore® |Cat# 20181065-2 |Size 10mL EA | $ 175.41

Specifications:

• Ligand: Glycine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B (Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of synaptic membrane proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glycine Separopore® |Cat# 20181065-3 |Size 25mL EA | $ 394.67

Specifications:

• Ligand: Glycine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B (Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h.
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of synaptic membrane proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

GTP Separopore® |Cat# 20181066-1 |Size 1mL EA | $ 45.09

Specifications:

• Ligand: Guanosine 5\'-Triphosphate (GTP)
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 50% Glycerol, 0.25M NaCL, 0.02% thimerosal.
• Application: For the purification of GTP dependent enzymes and proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

GTP Separopore® |Cat# 20181066-2 |Size 5mL EA | $ 155.73

Specifications:

• Ligand: Guanosine 5\'-Triphosphate (GTP)
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 50% Glycerol, 0.25M NaCL, 0.02% thimerosal.
• Application: For the purification of GTP dependent enzymes and proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

GTP Separopore® |Cat# 20181066-3 |Size 25mL EA | $ 621.47

Specifications:

• Ligand: Guanosine 5\'-Triphosphate (GTP)
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 50% Glycerol, 0.25M NaCL, 0.02% thimerosal.
• Application: For the purification of GTP dependent enzymes and proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Aspartic Acid Separopore® |Cat# 20181067-1 |Size 5mL EA | $ 131.85

Specifications:

• Ligand: L-Aspartic Acid
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of retinol, or other L-Aspartic Acid binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Aspartic Acid Separopore® |Cat# 20181067-2 |Size 10mL EA | $ 237.33

Specifications:

• Ligand: L-Aspartic Acid
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of retinol, or other L-Aspartic Acid binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Aspartic Acid Separopore® |Cat# 20181067-3 |Size 25mL EA | $ 453.96

Specifications:

• Ligand: L-Aspartic Acid
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of retinol, or other L-Aspartic Acid binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Glutamic Acid Separopore® |Cat# 20181068-1 |Size 5mL EA | $ 130.98

Specifications:

• Ligand: L-Glutamic Acid.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of membrane dicarboxylate proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Glutamic Acid Separopore® |Cat# 20181068-2 |Size 10mL EA | $ 237.45

Specifications:

• Ligand: L-Glutamic Acid.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of membrane dicarboxylate proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Glutamic Acid Separopore® |Cat# 20181068-3 |Size 25mL EA | $ 451.69

Specifications:

• Ligand: L-Glutamic Acid.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of membrane dicarboxylate proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Histidine Separopore® |Cat# 20181069-1 |Size 5mL EA | $ 70.47

Specifications:

• Ligand: L-Histidine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of histidine binding proteins.
• Storage temp. 2 to 8°C.
• Matrix active group Epoxy.
• Matrix spacer 12 atoms.
• Attachment Amino.
• Extent of labelling 1-2 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

L-Histidine Separopore® |Cat# 20181069-2 |Size 10mL EA | $ 126.85

Specifications:

• Ligand: L-Histidine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of histidine binding proteins.
• Storage temp. 2 to 8°C.
• Matrix active group Epoxy.
• Matrix spacer 12 atoms.
• Attachment Amino.
• Extent of labelling 1-2 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

L-Histidine Separopore® |Cat# 20181069-3 |Size 25mL EA | $ 285.41

Specifications:

• Ligand: L-Histidine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of histidine binding proteins.
• Storage temp. 2 to 8°C.
• Matrix active group Epoxy.
• Matrix spacer 12 atoms.
• Attachment Amino.
• Extent of labelling 1-2 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

L-Lysine Separopore® |Cat# 20181070-1 |Size 5mL EA | $ 93.75

Specifications:

• Ligand: L-Lysine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of lysine binding proteins from serum.
• Storage temp. 2 to 8°C.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment α-amino.
• Extent of labelling ~4 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Lysine Separopore® |Cat# 20181070-2 |Size 10mL EA | $ 167.4

Specifications:

• Ligand: L-Lysine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of lysine binding proteins from serum.
• Storage temp. 2 to 8°C.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment α-amino.
• Extent of labelling ~4 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Lysine Separopore® |Cat# 20181070-3 |Size 25mL EA | $ 351.45

Specifications:

• Ligand: L-Lysine
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B (Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of lysine binding proteins from serum.
• Storage temp. 2 to 8°C.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment α-amino.
• Extent of labelling ~4 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Phenylalanine Separopore® |Cat# 20181071-1 |Size 5mL EA | $ 98.75

Specifications:

• Ligand: L-Phenylalanine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment Amino.
• Extent of labelling 2-10 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Phenylalanine Separopore® |Cat# 20181071-2 |Size 10mL EA | $ 177.75

Specifications:

• Ligand: L-Phenylalanine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment Amino.
• Extent of labelling 2-10 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

L-Phenylalanine Separopore® |Cat# 20181071-3 |Size 25mL EA | $ 377.72

Specifications:

• Ligand: L-Phenylalanine.
• Particle size (range): 50-175µm.
• Molecular Weight range: 6x104 - 2x107.
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads).
• pH stability: 4-9.
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 2.0M NaCl, 0.02% thimerosal.
• Application: For the purification of phenylalanine binding proteins, such as phenylalanine hydroxylase.
• Matrix active group cyanogen bromide.
• Matrix spacer 1 atom.
• Attachment Amino.
• Extent of labelling 2-10 μmol per mL.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Maltose Separopore® |Cat# 20181072-1 |Size 1mL EA | $ 66.97

Specifications:

Ligand: Maltose.

Particle size (range): 50-175µm.

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads).

pH stability: 4-9.

Flow specifications: 70-140 cm/h.

Supplied as a suspension in 0.01 M phosphate buffer (pH 6.8), 0.15 M NaCl, 0.02% thimerosal.

Application: For the purification of maltose binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Maltose Separopore® |Cat# 20181072-2 |Size 5mL EA | $ 119.25

Specifications:

Ligand: Maltose.

Particle size (range): 50-175µm.

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads).

pH stability: 4-9.

Flow specifications: 70-140 cm/h.

Supplied as a suspension in 0.01 M phosphate buffer (pH 6.8), 0.15 M NaCl, 0.02% thimerosal.

Application: For the purification of maltose binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Maltose Separopore® |Cat# 20181072-3 |Size 10mL EA | $ 198

Specifications:

Ligand: Maltose.

Particle size (range): 50-175µm.

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B (Agarose 4% Beads).

pH stability: 4-9.

Flow specifications: 70-140 cm/h.

Supplied as a suspension in 0.01 M phosphate buffer (pH 6.8), 0.15 M NaCl, 0.02% thimerosal.

Application: For the purification of maltose binding proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Octyl Separopore® |Cat# 20181073-1 |Size 25mL EA | $ 86.52

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Octyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Octyl Separopore® |Cat# 20181073-2 |Size 50mL EA | $ 156.6

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Octyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Octyl Separopore® |Cat# 20181073-3 |Size 100mL EA | $ 287.88

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Octyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Phenyl Separopore® |Cat# 20181074-1 |Size 25mL EA | $ 191.25

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Phenyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Phenyl Separopore® |Cat# 20181074-2 |Size 50mL EA | $ 344.25

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Phenyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Phenyl Separopore® |Cat# 20181074-3 |Size 100mL EA | $ 593.1

Application: Hydrophobic chromatorgraphy.

Specifications:

Ligand: Phenyl group.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Poly - L - Lysine Separopore® |Cat# 20181075-1 |Size 5mL EA | $ 116.1

Specifications:

• Ligand: Poly-L-Lysine.
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..
• Application: For the purification of negatively charged proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Poly - L - Lysine Separopore® |Cat# 20181075-2 |Size 10mL EA | $ 208.98

Specifications:

• Ligand: Poly-L-Lysine.
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..
• Application: For the purification of negatively charged proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Poly - L - Lysine Separopore® |Cat# 20181075-3 |Size 25mL EA | $ 304.46

Specifications:

• Ligand: Poly-L-Lysine.
• Particle size (range): 50-175µm
• Molecular Weight range: 6x104 - 2x107
• Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)
• pH stability: 4-9
• Flow specifications: 70-140 cm/h
• Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal..
• Application: For the purification of negatively charged proteins.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glucose Separopore® |Cat# 20181076-1 |Size 1mL EA | $ 47.93

Specifications:

Ligand: ß-D-Glucose.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal.

Application: Affinity chromatography.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glucose Separopore® |Cat# 20181076-2 |Size 5mL EA | $ 201.15

Specifications:

Ligand: ß-D-Glucose.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal.

Application: Affinity chromatography.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glucose Separopore® |Cat# 20181076-3 |Size 10mL EA | $ 397.36

Specifications:

Ligand: ß-D-Glucose.

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Separopore® 4B-CL (Crosslinked Agarose 4% Beads)

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a suspension in 0.5 M NaCl, 0.02% thimerosal.

Application: Affinity chromatography.

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® 4B-Lyophilized |Cat# 20181077-1 |Size 2.5g EA | $ 128.98

Specifications

• 5 grams make 20 mL of gel.
• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH).
• Ligand couping method: Epoxy activation.
• Matrix: 4 % agarose bead.
• Binding capacity: > 5mg GST/ml drained gel.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 50-175µm.
• Storage: 2-8ºC.

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® 4B-Lyophilized |Cat# 20181077-2 |Size 5g EA | $ 249.86

Specifications

• 5 grams make 20 mL of gel.
• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH).
• Ligand couping method: Epoxy activation.
• Matrix: 4 % agarose bead.
• Binding capacity: > 5mg GST/ml drained gel.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 50-175µm.
• Storage: 2-8ºC.

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glutathione Separopore® 4B-Lyophilized |Cat# 20181077-3 |Size 25g EA | $ 538.76

Specifications

• 5 grams make 20 mL of gel.
• Reduced glutathione (GSH) covalently coupled to Separopore® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH).
• Ligand couping method: Epoxy activation.
• Matrix: 4 % agarose bead.
• Binding capacity: > 5mg GST/ml drained gel.
• Exclusion limit: approximately 2 x 107.
• Particle size (range): 50-175µm.
• Storage: 2-8ºC.

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Usage : bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

IgG (Mouse) Separopore®4B |Cat# 20181078-1 |Size 1mL EA | $ 78.55

IgG (Mouse) Separopore®

An affinity chromatography gel matrix consiting of mouse IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

IgG (Mouse) Separopore®4B |Cat# 20181078-2 |Size 5mL EA | $ 151.74

IgG (Mouse) Separopore®

An affinity chromatography gel matrix consiting of mouse IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

IgG (Mouse) Separopore®4B |Cat# 20181078-3 |Size 10mL EA | $ 241.39

IgG (Mouse) Separopore®

An affinity chromatography gel matrix consiting of mouse IgG bound to 4% agarose beads. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. This allows purification of anti-IgG antibodies. May also be used to immunoprecipitate Protein A produced by prokaryotic expression systems.

Specifications:

Size: 1 ml

Ligand: IgG

Ligand coupling method: CNBr activation

Matrix: 4%, beads

Particle size: 50-175µm

pH stability: 3-9

Storage: 2-8°C

AMP Separopore®, Lyophilized |Cat# 20181079-1 |Size 1g EA | $ 198

AMP Separopore®, Lyophilized

Specifications:

• Lyophilized powder
• 1g yields 4 -5 ml of gel
• Matrix: Highly cross-linked agarose beads, 4%
• Matrix attachment: N6
• Matrix activation: CNBr activation
• Ligand density: 2umol AMP/ml drained medium
• pH stability: 4-10
• Max Flow Rate: 75cm/h.
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents Physical stability: Negligible volume variation due to changes in pH or ionic strength

ATP Separopore®, Lyophilized |Cat# 20181080-1 |Size 1g EA | $ 178

Adenosine 5\'-triphosphate (ATP) Separapore™ (Lyophilized) for use in purification of ATP binding proteins.

Technical Specification:

• Lyophilized powder
• 1g yields 10-15ml of gel
• Matrix: Cross-linked Agarose beads, 4%.
• Particle size range: 50-175μm.
• Activation: Cyanogen bromide.
• Attachement: N-6 of the purine ring.
• Spacer: 11 atoms.
• Ligand: ATP.
• Ligand density: 2-5 μmol ATP/ml drained matrix.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Blue Sepharose® 6B-CL |Cat# 20182000-1 |Size 5mL EA | $ 44.28

Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). \"Reactive Dyes in Protein and Enzyme Technology.\" stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.

Blue Sepharose® 6B-CL |Cat# 20182000-2 |Size 25mL EA | $ 113.72

Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). \"Reactive Dyes in Protein and Enzyme Technology.\" stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.

Blue Sepharose® 6B-CL |Cat# 20182000-3 |Size 50mL EA | $ 215.77

Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). \"Reactive Dyes in Protein and Enzyme Technology.\" stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.

Blue Sepharose® 6B-CL |Cat# 20182000-4 |Size 100mL EA | $ 368.85

Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). \"Reactive Dyes in Protein and Enzyme Technology.\" stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.

Blue Sepharose® 6B-CL |Cat# 20182000-5 |Size 500mL EA | $ 1479.45

Blue Sepharose® CL-6B

• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD⁺ and NADP⁺), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Sepharose® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
  Reference:Clonis, Y.D., Atkinson, A., Bruton, C.J., and Lowe, C.R. (1987). \"Reactive Dyes in Protein and Enzyme Technology.\" stockton Press, New York.

Technical Specifications

• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Protocol for the Purification of Wnt Proteins

J Chromatogr. 1984 Jan 20;283:199-210.

Blue Sepharose behaved lika a strong cationic ion exchanger because of the negative charges of its dye ligand, Cibacron Blue. A study of the protein-Cibacron Blue interactions by phase partition and equilibrium dialysis revealed the presence of high-affinity binding sites both in the case of the (di)nucleoide-dependent enzymes entirely of alpha-helix or beta-sheet.  Preparative applications of these are illustrated in terms of the optimization of affinity chromatography experiments.

Con-A Sepharose® 4B |Cat# 20182001-1 |Size 1mL 2/EA | $ 51.68

Con-A Sepharose® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Sepharose® via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.
  Technical specifications
  • Ligand: Con-A
  • Ligand coupling method: CNBr activation
  • Ligand density: > 10mg/ml
  • Binding capacity: > 25 mg HRP/ml drained gel
  • Matrix: agarose, 4%
  • Exclusion limit: approximately 2 x 10⁷
  • Particle size (range): 50-175µm
  • Storage: 2-8ºC

Con-A Sepharose® 4B |Cat# 20182001-2 |Size 5mL EA | $ 69.5

Con-A Sepharose® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Sepharose® via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.
  Technical specifications
  • Ligand: Con-A
  • Ligand coupling method: CNBr activation
  • Ligand density: > 10mg/ml
  • Binding capacity: > 25 mg HRP/ml drained gel
  • Matrix: agarose, 4%
  • Exclusion limit: approximately 2 x 10⁷
  • Particle size (range): 50-175µm
  • Storage: 2-8ºC

Con-A Sepharose® 4B |Cat# 20182001-3 |Size 25mL EA | $ 205.5

Con-A Sepharose® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Sepharose® via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.
  Technical specifications
  • Ligand: Con-A
  • Ligand coupling method: CNBr activation
  • Ligand density: > 10mg/ml
  • Binding capacity: > 25 mg HRP/ml drained gel
  • Matrix: agarose, 4%
  • Exclusion limit: approximately 2 x 10⁷
  • Particle size (range): 50-175µm
  • Storage: 2-8ºC

Con-A Sepharose® 4B |Cat# 20182001-4 |Size 50mL EA | $ 374.46

Con-A Sepharose® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Sepharose® via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.
  Technical specifications
  • Ligand: Con-A
  • Ligand coupling method: CNBr activation
  • Ligand density: > 10mg/ml
  • Binding capacity: > 25 mg HRP/ml drained gel
  • Matrix: agarose, 4%
  • Exclusion limit: approximately 2 x 10⁷
  • Particle size (range): 50-175µm
  • Storage: 2-8ºC

Glutathione Sepharose® 4B |Cat# 20182003-2 |Size 10mL EA | $ 148.5

Glutathione Sepharose^® 4B

• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GST-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

(SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B |Cat# 20182003-3 |Size 25mL EA | $ 257.4

Glutathione Sepharose^® 4B

• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GST-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B |Cat# 20182003-4 |Size 50mL EA | $ 498.6

Glutathione Sepharose^® 4B

• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GST-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B |Cat# 20182003-5 |Size 100mL EA | $ 898.5

Glutathione Sepharose^® 4B

• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GST-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B |Cat# 20182003-6 |Size 1mL EA | $ 29.5

Glutathione Sepharose^® 4B

• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• Supplied as suspension in 20% ethanol.
• 1ml drained gel binds more than 5mg of recombinant GST-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.)

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B-Lyophilized |Cat# 20182004-1 |Size 5g EA | $ 387.5

Glutathione Sepharose^® 4B-Lyophilized

• 1 gram makes 10-15 mL of gel.
• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Glutathione Sepharose® 4B-Lyophilized |Cat# 20182004-2 |Size 1g EA | $ 149

Glutathione Sepharose^® 4B-Lyophilized

• 1 gram makes 10-15 mL of gel.
• Reduced glutathione (GSH) covalently coupled to Sepharose® via 10-carbon spacer arm.
• For affinity purification of proteins containing glutathione S-transferase (GST) fusion tag.
• 1ml drained gel binds more than 5mg of recombinant GS-protein.
• Ligand coupled: Reduced glutathione (GSH)
• Ligand couping method: Epoxy activation
• Matrix: 4 % agarose bead
• Binding capacity: > 5mg GST/ml drained gel
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 50-175µm
• Storage: 2-8ºC

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB.

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

AMP Sepharose®, Lyophilized |Cat# 20182005-1 |Size 1g EA | $ 237.5

AMP Sepharose^®

• Matrix: Highly cross-linked agarose, 4% 
• Ligand: AMP
• Matrix attachment: N-6
• Ligand density: 2umol AMP/ml drained medium
• Binding capacity: >10mg lactate dehydrogenase/ml
• pH stability: 4-10
• Max Flow Rate: 75cm/h.
• Supplied as lyophilized powder, stabilized with lactose
• Swelling: 1g swells approx. 5ml

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

AMP Sepharose®, Lyophilized |Cat# 20182005-2 |Size 3g EA | $ 387

AMP Sepharose^®

• Matrix: Highly cross-linked agarose, 4% 
• Ligand: AMP
• Matrix attachment: N-6
• Ligand density: 2umol AMP/ml drained medium
• Binding capacity: >10mg lactate dehydrogenase/ml
• pH stability: 4-10
• Max Flow Rate: 75cm/h.
• Supplied as lyophilized powder, stabilized with lactose
• Swelling: 1g swells approx. 4ml

Made with SEPHAROSE® chromatography media. SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Sepharose® 4B |Cat# 20182006-1 |Size 100mL EA | $ 106.2

Sepharose^® 4B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 4B |Cat# 20182006-2 |Size 500mL EA | $ 310.5

Sepharose^® 4B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 4B |Cat# 20182006-3 |Size 1L EA | $ 531.9

Sepharose^® 4B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquie).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 4% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 6B |Cat# 20182007-1 |Size 100mL EA | $ 128.45

Sepharose^® 6B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 6B |Cat# 20182007-2 |Size 500mL EA | $ 398.7

Sepharose^® 6B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 6B |Cat# 20182007-3 |Size 1L EA | $ 645.3

Sepharose^® 6B

A high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Larger volumes available at more competitive prices(enquire).

Specifications:

Particle size (range): 50-175µm

Molecular Weight range: 6x10⁴ - 2x10⁷

Matrix: Agarose 6% Beads

pH stability: 4-9

Flow specifications: 70-140 cm/h

Supplied as a slurry in 20% ethanol

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Ref : The Jour. of Biol. Chem. Vol. 281, NO. 31, pp. 21884-21891, August 4, 2006

Sepharose® 4B (Lyophilized), 50 mL Equivalent |Cat# 20182008-1 |Size 50mL EA | $ 64.16

Sepharose® 4B provided as lyophilized powder on resuspension swells to 50 mL gel. No additives added.

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Sepharose® 4B (Lyophilized), 100 mL Equivalent |Cat# 20182008-2 |Size 100mL EA | $ 109.81

Sepharose® 4B provided as lyophilized powder on resuspension swells to 100 mL gel. No additives added.

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Sepharose® 4B (Lyophilized), 500 mL Equivalent |Cat# 20182008-3 |Size 500mL EA | $ 359.5

Sepharose® 4B provided as lyophilized powder on resuspension swells to 500 mL gel. No additives added.

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).

Sepharose® 4B (Lyophilized), 1L Equivalent |Cat# 20182008-4 |Size 1L EA | $ 967.5

Sepharose® 4B provided as lyophilized powder on resuspension swells to 1L gel. No additives added.

SEPHAROSE® is a trademark of GE Healthcare Bio-Sciences AB (formerly known as Amersham Biosciences AB).