Blue Separopore® 6B-CL |Cat# 20181026-1 |Size 25mL | $ 112.72
Blue Separopore® CL-6B
• A Cibacron blue dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
Matrix: Highly cross-linked agarose, 6%
Ligand: Cibacron Blue F3G-A
Ligand coupling method: Triazine coupling
Ligand density: 8µmol Cibacron Blue/ml
Binding capacity: > 15mg bovine serum abumin/ml packed gel
Particle size (range): 45-165µ
pH stability: 3-13
Coupling conditions: pH 9-10,
Storage: 2-8ºC
Blue Separopore® 6B-CL |Cat# 20181026-2 |Size 50mL | $ 194.2
Blue Separopore® CL-6B
• A Cibacron blue dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
Matrix: Highly cross-linked agarose, 6%
Ligand: Cibacron Blue F3G-A
Ligand coupling method: Triazine coupling
Ligand density: 8µmol Cibacron Blue/ml
Binding capacity: > 15mg bovine serum abumin/ml packed gel
Particle size (range): 45-165µ
pH stability: 3-13
Coupling conditions: pH 9-10,
Storage: 2-8ºC
Blue Separopore® 6B-CL |Cat# 20181026-3 |Size 100mL | $ 331.97
Blue Separopore® CL-6B
• A Cibacron blue dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as suspension in 20% ethanol.
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
Matrix: Highly cross-linked agarose, 6%
Ligand: Cibacron Blue F3G-A
Ligand coupling method: Triazine coupling
Ligand density: 8µmol Cibacron Blue/ml
Binding capacity: > 15mg bovine serum abumin/ml packed gel
Particle size (range): 45-165µ
pH stability: 3-13
Coupling conditions: pH 9-10,
Storage: 2-8ºC
AMP Separopore®, Lyophilized |Cat# 20181079-2 |Size 3g | $ 348
AMP Separopore®, Lyophilized
Specifications:
- Lyophilized powder
- 1g yields 4 -5 ml of gel
- Matrix: Highly cross-linked agarose beads, 4%
- Matrix attachment: N6
- Matrix activation: CNBr activation
- Ligand density: 2umol AMP/ml drained medium
- pH stability: 4-10
- Max Flow Rate: 75cm/h.
- Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents Physical stability: Negligible volume variation due to changes in pH or ionic strength
Goat Anti-Rabbit IgG (H+L)-Red Separopore® |Cat# 20181081-1 |Size 1mL | $ 87.7
Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore®. Affinity purified goat anti-mouse IgG bound to Separopore® offers the advantage of increased sensitiveity for rabbit IgG without cross-reactivity to other substance present on the membrane or cell surface.
Application: For use in immunoprecipitation and Co-immunoprecipitation studies.
The antibody separopSeparopore® is supplied as a suspension (1:1) in 0.01M PBS buffer (pH 7.4) containing 15mM sodium azide as a preservative.
Goat Anti-Rabbit IgG (H+L)-Red Separopore® |Cat# 20181081-2 |Size 2mL | $ 135
Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore®. Affinity purified goat anti-mouse IgG bound to Separopore® offers the advantage of increased sensitiveity for rabbit IgG without cross-reactivity to other substance present on the membrane or cell surface.
Application: For use in immunoprecipitation and Co-immunoprecipitation studies.
The antibody separopSeparopore® is supplied as a suspension (1:1) in 0.01M PBS buffer (pH 7.4) containing 15mM sodium azide as a preservative.
Goat Anti-Mouse IgG (FC)-Red Separopore® |Cat# 20181082-1 |Size 1mL | $ 87.7
Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore®. The antibody preparation is specific for the Fc fragment of mouse IgG when tested against purified mouse IgA, IgG, IgM, Fc and kappa and lamda light chains. Affinity purified goat anti-mouse IgG bound to Separopore® offers the advantage of increased sensitiveity for mouse IgG without cross-reactivity to other substance present on the membrane or cell surface.
Application: For use in immunoprecipitation and Co-immunoprecipitation studies.
The antibody separopSeparopore® is supplied as a suspension (1:1) in 0.01M PBS buffer (pH 7.4) containing 15mM sodium azide as a preservative.
Goat Anti-Mouse IgG (FC)-Red Separopore® |Cat# 20181082-2 |Size 2mL | $ 135
Affinity isolated antibody is coupled to cyanogen bromide-activated Separopore®. The antibody preparation is specific for the Fc fragment of mouse IgG when tested against purified mouse IgA, IgG, IgM, Fc and kappa and lamda light chains. Affinity purified goat anti-mouse IgG bound to Separopore® offers the advantage of increased sensitiveity for mouse IgG without cross-reactivity to other substance present on the membrane or cell surface.
Application: For use in immunoprecipitation and Co-immunoprecipitation studies.
The antibody separopSeparopore® is supplied as a suspension (1:1) in 0.01M PBS buffer (pH 7.4) containing 15mM sodium azide as a preservative.
Blue Separopore® 6B-CL, Lyophylized |Cat# 20181083-1 |Size 5g | $ 113.4
Blue Separopore® CL-6B
• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as lyophylized powder (1 g swells to approx. 4 ml).
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC
Blue Separopore® 6B-CL, Lyophylized |Cat# 20181083-2 |Size 10g | $ 213.87
Blue Separopore® CL-6B
• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as lyophylized powder (1 g swells to approx. 4 ml).
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC
Blue Separopore® 6B-CL, Lyophylized |Cat# 20181083-3 |Size 25g | $ 364.2
Blue Separopore® CL-6B
• A dye-ligand affinity matrix for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.
• The ligand, Cibacron Blue F3G-A, is covalently coupled to Separopore® via chlorotriazine ring.
• Supplied as lyophylized powder (1 g swells to approx. 4 ml).
• Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool.
Technical Specifications
• Matrix: Highly cross-linked agarose, 6%
• Ligand: Cibacron Blue F3G-A
• Ligand coupling method: Triazine coupling
• Ligand density: 8µmol Cibacron Blue/ml
• Binding capacity: > 15mg bovine serum abumin/ml packed gel
• Particle size (range): 45-165µ
• pH stability: 3-13
• Coupling conditions: pH 9-10,
• Storage: 2-8ºC
CDI-activated Separopore® 6B |Cat# 20181084-1 |Size 5mL | $ 58.5
CDI-activated Separopore®6B
- Pre-activated medium for immobilization of ligands containing primary amines.
- N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
- No charge is introduced into the matrix after the coupling reaction.
- No ligand leakage after coupling.
- Superior stability: activated matrix is much more resistant to hydrolysis.
- Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
- Coupling reaction can also be carried out in organic solvents.
- Supplied as gel suspension in acetone.
Technical Specifications
- Group to be coupled: -NH2
- Chemical linkage of ligand: stable amide carbamate linkage
- Matrix: cross-linked agarose, 6% beads
- Particle size (range): 50-175µm
- Fractionation range (MW): 10,000-400,000
- pH stability: 11 (ligand dependent)
- Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
CDI-activated Separopore® 6B |Cat# 20181084-2 |Size 25mL | $ 165
CDI-activated Separopore®6B
- Pre-activated medium for immobilization of ligands containing primary amines.
- N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
- No charge is introduced into the matrix after the coupling reaction.
- No ligand leakage after coupling.
- Superior stability: activated matrix is much more resistant to hydrolysis.
- Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
- Coupling reaction can also be carried out in organic solvents.
- Supplied as gel suspension in acetone.
Technical Specifications
- Group to be coupled: -NH2
- Chemical linkage of ligand: stable amide carbamate linkage
- Matrix: cross-linked agarose, 6% beads
- Particle size (range): 50-175µm
- Fractionation range (MW): 10,000-400,000
- pH stability: 11 (ligand dependent)
- Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
CDI-activated Separopore® 6B |Cat# 20181084-3 |Size 100mL | $ 436.04
CDI-activated Separopore®6B
- Pre-activated medium for immobilization of ligands containing primary amines.
- N,N\'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
- No charge is introduced into the matrix after the coupling reaction.
- No ligand leakage after coupling.
- Superior stability: activated matrix is much more resistant to hydrolysis.
- Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
- Coupling reaction can also be carried out in organic solvents.
- Supplied as gel suspension in acetone.
Technical Specifications
- Group to be coupled: -NH2
- Chemical linkage of ligand: stable amide carbamate linkage
- Matrix: cross-linked agarose, 6% beads
- Particle size (range): 50-175µm
- Fractionation range (MW): 10,000-400,000
- pH stability: 11 (ligand dependent)
- Coupling conditions: pH 8.5-11, 4-25ºC, 6-24 h.
Protein-A Sepharose/Salmon Sperm DNA |Cat# 20182011-1 |Size 2.5mL | $ 217
Protein A Sepharose/Salmon Sperm DNA
Description: Protein A covalently bound by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purfy IgG fractions from serum or ascites fluid. Suitable for use in chromatin immunoprecipitation (ChIPs) assays. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Valuated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones, followed by antio-Acetyl Histone H4.
- 10mM tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
- Storage: 1 year at 4°C.
- Packaging: 2.5mL packed beads provided as a 50% gel slurry.
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