Saturday, January 1, 2011

Apoptosis Detection Kits

Apoptotic DNA-ladder kit |Cat# 20710000-1 |Size 100preps KT | $ 198


  • The Apoptotic DNA-Ladder kit contains all of the reagents necessary for the preparation of an apoptotic DNA ladder.
  • The protocol involves cell lysis, removal of cellular debris, and is followed by precipitation of DNA.
  • Nucleosomal DNA is detected after standard 1.8% agarose gel electrophoresis.

CasPASE 1, 4, 5 Apoptosis Assay Kit, 50 Assays |Cat# 20710001-1 |Size 50ASSAY KT | $ 198


CasPase™ 1 4 5 Assay Kit

bioWorld provides reagents for the detection of apoptosis by measuring the caspase activity- an early indicator of apoptosis. The assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Procedure uses an AFC-substrate that exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

CasPase™ reaction releases AFC free dye from the substrate, which undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 assays
  • CasPASE 1, 4, 5 Apoptosis Assay Kit,100 Assays |Cat# 20710001-2 |Size 100ASSAY KT | $ 263


    CasPase™ 1 4 5 Assay Kit

    bioWorld provides reagents for the detection of apoptosis by measuring the caspase activity- an early indicator of apoptosis. The assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Procedure uses an AFC-substrate that exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    CasPase™ reaction releases AFC free dye from the substrate, which undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 assays
  • CasPASE 2, Apoptosis Assay Kit,50 Assays |Cat# 20710002-1 |Size 50ASSAY KT | $ 198


    CasPase™ -2, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 Assays
  • CasPASE 2, Apoptosis Assay Kit,100 Assays |Cat# 20710002-2 |Size 100ASSAY KT | $ 263


    CasPase™ -2, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 Assays
  • CasPASE 3, 7, 10 Apoptosis Assay Kit,50 Assays |Cat# 20710003-1 |Size 50ASSAY KT | $ 198


    CasPASE™ 3 7 10, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 Assays
  • CasPASE 3, 7, 10 Apoptosis Assay Kit,100 Assays |Cat# 20710003-2 |Size 100ASSAY KT | $ 263


    CasPase™ -3, 7, 10 Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 Assays
  • CasPASE 6 Apoptosis Assay Kit,50 Assays |Cat# 20710004-1 |Size 50ASSAY KT | $ 198


    CasPase™ 6, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 Assays
  • CasPASE 6 Apoptosis Assay Kit,100 Assays |Cat# 20710004-2 |Size 100ASSAY KT | $ 263


    CasPase™ 6, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 Assays
  • CasPASE 8 Apoptosis Assay Kit,50 Assays |Cat# 20710005-1 |Size 50ASSAY KT | $ 198


    CasPase™ 8, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 Assays
  • CasPASE 8 Apoptosis Assay Kit,100 Assays |Cat# 20710005-2 |Size 100ASSAY KT | $ 263


    CasPase™ 8, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 Assays
  • CasPASE 9 Apoptosis Assay Kit,50 Assays |Cat# 20710006-1 |Size 50ASSAY KT | $ 198


    CasPase™ 9, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 50 Assays
  • CasPASE 9 Apoptosis Assay Kit,100 Assays |Cat# 20710006-2 |Size 100ASSAY KT | $ 263


    CasPase™ 9, Assay Kit

    Provides reagents for the detection of apoptosis by measuring the caspase activity - an early indicator of apoptosis. Assay can be monitored with either a fluorescence reader, a spectrophotometer or microtiter plate reader. Uses AFC-substrate which exhibits both fluorescence and absorbance spectral shifts between the substrate-conjugate and the free dye (AFC).

    During the CasPase reaction, the substrate releases AFC free dye and undergoes both a fluorecence and absorbance shift. Since AFC-substrate exhibits dual-mode detection capability, arbitrary fluorescence values can be standardized to an optical density value. When measuring fluorescence, the assay reaction is excited at 390-400nm. If the assay is measured with a spectrophotometer or microtiter plate reader, the absorbance is measured at 390-400.

    Specifications:

  • Assay can be conveniently adapted for high-throughput 96-well format.
  • Assay system may be used with purified enzyme preparations, cell extracts or tissue lysates.
  • Supplied with necessary assay buffers, enzyme specific AFC-substrate, free dye (AFC) and caspase inhibitor Z-VAD-FMK for establishing proper positive and negative controls and standards.
  • 100 Assays
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