Bio LC Prepacked Affinity Columns

Arachis hypogaea (Peanut) Separopore® |Cat# 20120000-1 |Size 10mL EA | $ 167.4

Peanut agglutinin (PNA, Peanut Agglutinin) is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (b-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the “T-antigen”) is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding has been employed as a measure of cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte human subpopulations and animals, and to measure the levels of lymphoid cell populations in many diseases. Presence of Ca⁺⁺ can enhance PNA binding.

Specifications:

• Form: Supplied in 1% NaCl containing 0.04% sodium azide as preservative.
• Matrix: Agarose, 6% Macrobeads.
• Bead size range: 250µm-400 µm diameter.
• Ligand source:  Arachis hypogaea (peanut).
• Ligand coupling method: CNBr activation.
• Ligand Concentration: 5 mg Peanut lectin/ml drained gel.
• Binding Capacity: 0.5mg/ml.
• Inhibiting/Eluting Sugar: 200 mM galactose.
• Carbohydrate for Elution: 0.1M lactose or 0.2M galactose in buffer. 10-50mM N-acetylgalactosamine may also be used.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Arachis hypogaea (Peanut) Separopore® |Cat# 20120000-2 |Size 25mL EA | $ 298.5

Peanut agglutinin (PNA, Peanut Agglutinin) is a 110,000 molecular weight lectin composed of four identical subunits of approximately 27,000 daltons each. PNA binds preferentially to a commonly occurring structure, galactosyl (b-1,3) N-acetylgalactosamine. This carbohydrate sequence (called the “T-antigen”) is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids.

PNA is useful in distinguishing between normal and tumor tissues and in assessing malignancy in transitional mucosa. In addition, PNA binding has been employed as a measure of cellular maturity in lymphoid tissues, to distinguish a variety of lymphocyte human subpopulations and animals, and to measure the levels of lymphoid cell populations in many diseases. Presence of Ca⁺⁺ can enhance PNA binding.

Specifications:

• Form: Supplied in 1% NaCl containing 0.04% sodium azide as preservative.
• Matrix: Agarose, 6% Macrobeads.
• Bead size range: 250µm-400 µm diameter.
• Ligand source:  Arachis hypogaea (peanut).
• Ligand coupling method: CNBr activation.
• Ligand Concentration: 5 mg Peanut lectin/ml drained gel.
• Binding Capacity: 0.5mg/ml.
• Inhibiting/Eluting Sugar: 200 mM galactose.
• Carbohydrate for Elution: 0.1M lactose or 0.2M galactose in buffer. 10-50mM N-acetylgalactosamine may also be used.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Artocarpus integrifolia Separopore® |Cat# 20120001-1 |Size 0.8mL EA | $ 95.6

Specifications:

• M.W. 52kD
• Source Artocarpus altilis (Jackfruit) , A. altilis \'Seminifera\' (Breadfruit).
• Form Supplied as a lyophilized powder in PBS, pH 7.2-7.4. Labeled with Biotin.
• Reconstitution Reconstitute with buffer to 1mg/ml.
• Biological Activity Less than 0.5ug/ml will agglutinate neuraminidase treated human erythrocytes.
• Specificity Primary affinity for terminal a-galactose residues and T-antigen which has a terminal b-galactose residue. It does not bind to b-galactose structures as in lactose.
• Carbohydrate Specificity B-galactose.
• Storage & stability Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Purity Affinity purified.
• Applications Suitable for use in purification of human serum IgA and other immunoglobulins.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Artocarpus integrifolia Separopore® |Cat# 20120001-2 |Size 3mL EA | $ 131.6

Specifications:

• M.W. 52kD
• Source Artocarpus altilis (Jackfruit) , A. altilis \'Seminifera\' (Breadfruit).
• Form Supplied as a lyophilized powder in PBS, pH 7.2-7.4. Labeled with Biotin.
• Reconstitution Reconstitute with buffer to 1mg/ml.
• Biological Activity Less than 0.5ug/ml will agglutinate neuraminidase treated human erythrocytes.
• Specificity Primary affinity for terminal a-galactose residues and T-antigen which has a terminal b-galactose residue. It does not bind to b-galactose structures as in lactose.
• Carbohydrate Specificity B-galactose.
• Storage & stability Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Purity Affinity purified.
• Applications Suitable for use in purification of human serum IgA and other immunoglobulins.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Glycine max (soybean) Separopore® |Cat# 20120002-1 |Size 0.8mL EA | $ 140.6

Glycine max (soybean) Separopore® |Cat# 20120002-2 |Size 3mL EA | $ 163.1

Lens culinaris (lentil) Separopore® |Cat# 20120003-1 |Size 0.8mL EA | $ 97.85

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Specifications

• M.W. 49kD.
• Source Lentil Seed.
• Form Supplied as a liquid in 0.05M Tris, 0.15M NaCl, pH 7.0-7.2.
• Biological Activity 50-200ug/ml will agglutinate type O human erythrocytes. 2-5ug/ml will agglutinate neuraminidase treated cells.
• Inhibitory Carbohydrate D-Mannose and D-Glucose. Fucose linked a(1,6) to core GlcNAc of N-linked glycopeptides to determine lectin activity.
• Storage & stability Extended stability when stored at -20ºC.
• Quality control SDS-PAGE.
• Concentration ~10mg/ml.
• Note Addition of mM concentrations of Mn+2 and Ca+2 may be added to reconstitution buffer to enhance hemagglutination.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Lens culinaris (lentil) Separopore® |Cat# 20120003-2 |Size 3mL EA | $ 215.96

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Specifications

• M.W. 49kD.
• Source Lentil Seed.
• Form Supplied as a liquid in 0.05M Tris, 0.15M NaCl, pH 7.0-7.2.
• Biological Activity 50-200ug/ml will agglutinate type O human erythrocytes. 2-5ug/ml will agglutinate neuraminidase treated cells.
• Inhibitory Carbohydrate D-Mannose and D-Glucose. Fucose linked a(1,6) to core GlcNAc of N-linked glycopeptides to determine lectin activity.
• Storage & stability Extended stability when stored at -20ºC.
• Quality control SDS-PAGE.
• Concentration ~10mg/ml.
• Note Addition of mM concentrations of Mn+2 and Ca+2 may be added to reconstitution buffer to enhance hemagglutination.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ulex europaeus Separopore® |Cat# 20120004-1 |Size 3mL EA | $ 249

Specifications

• Form lyophilized powder.
• Storage & stability 2-8°C.
• Bichem/physiol actions Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
• Linkage conjugates Prepared from affinity purified lectin.
• Potency <8 μg/mL agglutination activity.
• Composition protein ≥80% biuret.
• Total impurities salt, essentially free.

Note Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Ulex europaeus Separopore® |Cat# 20120004-2 |Size 0.8mL EA | $ 98

Specifications

• Form lyophilized powder.
• Storage & stability 2-8°C.
• Bichem/physiol actions Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
• Linkage conjugates Prepared from affinity purified lectin.
• Potency <8 μg/mL agglutination activity.
• Composition protein ≥80% biuret.
• Total impurities salt, essentially free.

Note Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Concanavalin A (Con-A) Separopore® |Cat# 20120005-1 |Size 0.8mL 2/PK | $ 41.34

Con-A Separopore® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Separopore®™ via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.

Technical specifications

• Ligand: Con-A
• Ligand coupling method: CNBr activation
• Ligand density: > 10mg/ml
• Binding capacity: > 25 mg HRP/ml drained gel
• Matrix: agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-150µm

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Concanavalin A (Con-A) Separopore® |Cat# 20120005-2 |Size 3mL EA | $ 62.01

Con-A Separopore® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Separopore®™ via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.

Technical specifications

• Ligand: Con-A
• Ligand coupling method: CNBr activation
• Ligand density: > 10mg/ml
• Binding capacity: > 25 mg HRP/ml drained gel
• Matrix: agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-150µm

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Concanavalin A (Con-A) Separopore® |Cat# 20120005-3 |Size 5mL EA | $ 85.65

Con-A Separopore® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Separopore®™ via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.

Technical specifications

• Ligand: Con-A
• Ligand coupling method: CNBr activation
• Ligand density: > 10mg/ml
• Binding capacity: > 25 mg HRP/ml drained gel
• Matrix: agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-150µm

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Concanavalin A (Con-A) Separopore® |Cat# 20120005-4 |Size 25mL EA | $ 148.5

Con-A Separopore® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Separopore® ™ via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.

Technical specifications

• Ligand: Con-A
• Ligand coupling method: CNBr activation
• Ligand density: > 10mg/ml
• Binding capacity: > 25 mg HRP/ml drained gel
• Matrix: agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-150µm

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Concanavalin A (Con-A) Separopore® |Cat# 20120005-5 |Size 25mL EA | $ 184.95

Con-A Separopore® 4B

• Affinity matrix for purification of macromolecules containing α-D-mannosyl or α-D-glucosyl groups such as glycoproteins, glycolipids and polysaccharides.
• The ligand, concanavalin A (Con-A), is covalently coupled to Separopore®™ via CNBr activation.
• Ideal for isolation of cell surface glycoproteins from detergent solubilized membranes.
• Supplied as suspension in 20% ethanol.

Technical specifications

• Ligand: Con-A
• Ligand coupling method: CNBr activation
• Ligand density: > 10mg/ml
• Binding capacity: > 25 mg HRP/ml drained gel
• Matrix: agarose, 4% beads
• Exclusion limit: approximately 2 x 10⁷
• Particle size (range): 45-150µm

Usage :  bioWORLD\'s products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Mini-Columns, 0.5 ml |Cat# 21160000-1 |Size 0.5mL 200/CS | $ 67.36

• 0.5 ml Microspin columns, 200/case
• molded in polypropylene
• features a HDPE filter of medium porosity (45-90 micron)